高效液相色谱测定甾体化合物的微生物转化过程

DETERMINATION OF MICROBIAL TRANSFORMATION PRODUCTS OF STEROIDS BY HPLC

对甾体的几种典型微生物转化反应如11α-,11β-,16α-羟基化、1,2位脱氢、A环芳构化以及不对称还原等产物有效地进行反相高效液相色谱分离。同时还定量测定微生物1,2位脱氢所形成的16β-甲基-17α-羟基孕-1,4,9(11)-三烯-3,20-二酮。

This paper describes the reversed phase HPLC determination ofsome microbial transformation products of steroids,including 11α-,11β-,16α-hydroxylation,1,2-dehydrogenation,ring A aromatization and asymmetricreduction.