葛根芩连汤中各成分在正常与大肠湿热证模型大鼠的肠吸收差异研究

Differences in intestinal absorption of Gegen Qinlian Decoction between normal rats and rats with large intestinal damp-heat syndrome

为比较正常大鼠与大肠湿热证模型大鼠对葛根芩连汤中指标成分的肠吸收差异,并从吸收环节探讨该复方临床治疗大肠湿热证的合理性,该研究将低、中、高3个浓度的葛根芩连汤分别在正常大鼠和大肠湿热证模型大鼠进行离体外翻肠囊法肠试验,采用HPLC检测肠外翻样品中10个指标成分的含量,计算各成分在各肠段的累积吸收量(Q/μg)和吸收速率(Ka),并进行对比。结果发现正常鼠和模型鼠对同质量浓度的葛根芩连汤吸收程度不同。大肠湿热证模型大鼠使葛根芩连汤中的多数成分在不同肠段的肠吸收均有一定程度增加。其中200 g·L-1质量浓度葛根芩连汤中各组分的累积吸收量均在模型鼠空肠最高,除大豆苷和黄芩苷外均在回肠、十二指肠、结肠中吸收依次降低;比较吸收速率常数,各组分在十二指肠与空肠吸收均显著增加(P<0.01),在回肠吸收均显著性降低(P<0.01),另外葛根素、大豆苷、甘草苷、黄连碱及小檗碱在结肠的吸收选择性增加。因此提示在进行吸收效应相关的成分研究时,宜采用病理模型动物,可真正为葛根芩连汤对症治疗大肠湿热证奠定研究基础。

The study aimed to compare the difference in intestinal absorption of the components of Gegen Qinlian Decoction between normal rats and those with large intestinal damp-heat syndrome in the pathological state,in order to explore the rational application of Gegen Qinlian Decoction in the treatment of large intestinal damp-heat syndrome.Puerarin,daidzin,liquiritin,scutellarin,baicalin,wogonoside,coptisine,jatrorrhizine,berberine and palmatine were used as the detection indexes in the in vitro everted gut sacs absorption experiment.The cumulative absorption amount(Q/μg)and the absorption rate(Ka)of each component in each intestine segment were calculated and compared.It was found that the absorption of each component in different intestinal segments were linear absorption,with R2 greater than 0.9,which conformed to the zero-order absorption rate.There were differences between normal rats and model rats in the absorption of the components in Gegen Qinlian Decoction with the same concentration.Intestinal absorption of most components of Gegen Qinlian Decoction in the model of large intestinal damp-heat syndrome increased to some extent.The components of Gegen Qinlian Decoction with the concentration of 200 g·L-1 had the highest absorption in the jejunum of the model rats,and the absorption in the ileum,duodenum and colon successively decreased except daidzin and baicalin.In terms of the absorption rate constant,the absorption in the duodenum and jejunum were significantly increased(P<0.01)compared with normal rats,and the absorption in the ileum was significantly decreased(P<0.01)compared with normal rats.In addition,the absorption of puerarin,daidzin,glycyrrhizin,coptisine and berberine increased selectivity in the colon.Therefore,pathological model animals were recommended in the study of the components relating to absorption effect,in order to really lay a research foundation for the symptomatic treatment of large intestinal damp-heat syndrome.