摘要
目的探讨过表达miR-202对甲状腺癌细胞恶性表型的影响及作用机制。方法选取2017年1月至2018年12月间深圳大学附属第一医院收治的行手术治疗的甲状腺癌患者的癌组织及癌旁正常组织各32例,用qRT-PCR检测miR-202在甲状腺癌组织中的表达,对甲状腺癌细胞进行miR-202模拟物转染或miR-202模拟物与ROCK1表达载体共转染,采用qRT-PCR检测转染效率,用CCK8实验和Caspase-3实验检测转染后甲状腺癌细胞的增殖和凋亡能力。采用qRT-PCR检测miR-202模拟物转染后ROCK1、E-cadherin、Twist、N-cadherin和MMP2的表达。采用双荧光素酶报告基因法检测ROCK1与miR-202之间的靶向关系。结果 miR-202在甲状腺癌组织中下调,miR-202的上调可抑制甲状腺癌细胞增殖,促进甲状腺癌细胞凋亡。miR-202上调促进E-cadherin表达,抑制N-cadherin、Twist和MMP2的表达。ROCK1是miR-202的靶基因,miR-202通过靶向ROCK1调控甲状腺癌细胞的增殖和凋亡能力及相关基因表达。结论 miR-202通过靶向ROCK1基因调节甲状腺癌细胞的增殖和凋亡。
Objective To study the effect of overexpression of miR-202 on the malignant phenotype of thyroid cancer cells and its mechanism. Methods Thirty-two thyroid cancer tissues and 32 normal paracancerous tissues were collected from patients with thyroid cancer who underwent surgery at The First Affiliated Hospital of Shenzhen University from January 2017 to December 2018. qRT-PCR was used to detect the expression of miR-202 in thyroid carcinoma tissues. Transfection with miR-202 mimics or co-transfection with miR-202 mimics and ROCK1 expression vector was performed on the thyroid cancer cells. Transfection efficiency was detected by qRT-PCR. CCK8 and Caspase-3 assay were used to detect the proliferation and apoptosis of thyroid cancer cells after transfection. qRT-PCR was conducted to research the expression of ROCK1,E-cadherin,Twist,N-cadherin and MMP2. Dual luciferase reporter assay was performed to detect the targeted relationship between ROCK1 and miR-202. Results The expression of miR-202 in thyroid cancer tissues and cells was significantly down-regulated. Up-regulation of miR-202 expression inhibited the proliferation and promoted the apoptosis of thyroid cancer cells. Up-regulated expression of miR-202 promoted the expression of E-cadherin and inhibited the expression of N-cadherin,Twist,and MMP2. miR-202-5 p targeted ROCK1. miR-202 regulated proliferation,apoptosis,and the expression of related genes in thyroid cancer cells by targeting ROCK1 expression. Conclusion miR-202 regulates proliferation and apoptosis of thyroid cancer cells by targeting the gene ROCK1.
作者
吴恢升
吴妹
曾海勇
王先明
WU Hui-sheng;WU Mei;ZENG Hai-yong;WANG Xian-ming(Department of Breast and Thyroid Surgery,The First Affiliated Hospital of Shenzhen University,Shenzhen 518000,China;Department of Hyperbaric Oxygen,The First Affiliated Hospital of Shenzhen University,Shenzhen 518000,China;Department of Endocrine Diseases,The First Affiliated Hospital of Shenzhen University,Shenzhen 518000,China)
出处
《中国肿瘤临床与康复》
2020年第3期261-265,共5页
Chinese Journal of Clinical Oncology and Rehabilitation
基金
广东省医学科学技术研究基金项目(B2016078)
深圳市科技计划项目(201302083).
