盘龙七片对膝骨关节炎大鼠软骨细胞凋亡的抑制及其作用机理

Inhibitory Effect of Panlongqi Tablet on Chondrocyte Apoptosis in Rats with Knee Osteoarthritis and Its Mechanism

目的:分析中成药盘龙七片对膝骨关节炎大鼠软骨细胞凋亡的抑制作用,并探讨其作用机理。方法:采用膝关节穿刺注射胶原酶法制备膝骨关节炎大鼠模型,检测不同剂量盘龙七片处理后大鼠膝关节肿胀及外周血中炎性因子IL-1β和IL-6水平变化情况;同时检测盘龙七片对炎症因子诱导的软骨细胞凋亡及Bcl-2和Caspase-3表达水平、GSK3β磷酸化水平的影响。结果:高、低剂量盘龙七片均可降低膝骨关节炎大鼠膝关节肿胀程度及炎症因子IL-1β和IL-6水平。盘龙七片处理后,大鼠膝关节软骨组织中及体外炎症因子诱导的软骨细胞中凋亡抑制因子Bcl-2的表达水平明显增高,Caspase-3表达量则显著降低,激酶GSK3β磷酸化水平及细胞凋亡水平明显下降,差异有统计学意义(P<0.05)。以GSK3β激活剂LY294002作用于细胞后,可明显减弱盘龙七片对软骨细胞凋亡的抑制作用。结论:盘龙七片可通过抑制GSK3β激酶活性,缓解膝关节软骨细胞的凋亡,进而对膝骨关节炎发挥治疗作用。

Objective:To analysis the inhibitory effect of Panlongqi tablet on chondrocyte apoptosis in rats with knee osteoarthritis and its potential mechanism.Methods:Model rats of knee osteoarthritis were established by injection of collagenase into knee joint.The swelling of knee joint and the levels of IL-1βand IL-6 in peripheral blood of rats were tested after treatment with different doses of Panlongqi tablet.At the same time,the effects of Panlongqi tablet on chondrocyte apoptosis,Bcl-2 and Caspase-3 expression level,GSK3βkinase level induced by inflammatory factors were detected.Results:The degree of knee swelling and the levels of inflammatory factors IL-1βand IL-6 in rats with knee osteoarthritis were reduced by both high and low dose of Panlongqi tablet.Besides,the expression of Bcl-2 and Caspase-3 in articular cartilage and chondrocytes induced by inflammatory factors in vitro were significantly increased,whereas the levels of Caspase-3 and apoptosis as well as GSK3βkinase were significantly decreased(P<0.05).After treatment with GSK3βactivator LY294002,the inhibitory effect of Panlongqi tablet on chondrocyte apoptosis was significantly weakened.Conclusion:Panlongqi tablet plays a role in the treatment of knee osteoarthritis by inhibiting the activity of GSK3βkinase,alleviating the apoptosis of chondrocytes in knee joint.