连翘木脂素及半日花烷型二萜研究

Lignans and labdane diterpenoids from the fruits of Forsythia suspensa Thunb.vahl

目的研究连翘(Forsythia suspensa Thunb vahl)抗炎活性部位中的化学成分进行。方法综合运用硅胶柱色谱、Sephadex LH-20柱色谱及高效制备色谱等方法对连翘中的化合物进行分离纯化,并根据理化性质和波谱数据对其化学结构进行鉴定。结果从连翘体积分数95%乙醇提取物的氯仿部位分离得到7个木脂素、5个半日花烷型二萜和2个其他类化合物,分别鉴定为:连翘脂素(1),松酯醇(2),表松脂酚(3),松脂素单甲醚(4),表桉叶明(5),异落叶松脂醇(6),3,3′-二甲氧基4,4′,9-三羟基-7,9′-环氧木脂素-7′-酮(7),19-hydroxy-ent-labda-8(17),13E-dien-15-oic acid(8),ent-labda-8(17),13Z-dien-15,19-dioic acid(9),ent-labda-8(17),13E-dien-15,19-dioic acid(10),3β-hydroxy-8(17),13E-labdadien-15-oic acid(11),enantio-labda-8(20),13E-dien-15,18-dioic acid(12),麦珠子酸(13),白桦脂酸(14)。结论化合物5、9、10为首次从连翘中发现,也均为首次从连翘属植物中发现。

To study the chemical constituents from the active portion of Forsythia suspensa Thunb.vahl.,fourteen compounds including seven lignans,five labdane diterpenoids and two other compounds were isolated and purified through preparative HPTLC and column chromatography over silica gel and Sephadex LH-20.Based on the physico-chemical properties and spectral data,their structures were identified as phillygenol(1),pinoresinol(2),epipinoresinol(3),pinoresinol monomethyl ether(4),epieudesmin(5),isolariciresinol(6),3,3′-dimethoxy-4,4′,9-trihydroxy-7,9′-epoxylignan-7′-one(7),19-hydroxy-ent-labda-8(17),13E-dien-15-oic acid(8),ent-labda-8(17),13Z-dien-15,19-dioic acid(9),ent-labda-8(17),13E-dien-15,19-dioic acid(10),3β-hydroxy-8(17),13E-labdadien-15-oic acid(11),enantio-labda-8(20),13E-dien-15,18-dioic acid(12),alphitolic acid(13)and betulinic acid(14).For the first time,compounds 5,9 and 10 were isolated from this species and Forsythia plants.