驴乳溶菌酶原核表达及活性检测

Prokaryotic expression and activity determination of donkey milk lysozyme

为探究驴乳源溶菌酶特性,本研究人工合成了驴乳溶菌酶基因DKLYSC1,并连入原核表达载体pET32a(+)中经诱导、表达,通过SDS-PAGE、western blot及比浊法检测重组溶菌酶表达及活性。结果显示,重组溶菌酶主要以包涵体形式高效表达,分子质量约31 ku;纯化的重组蛋白可以与抗His标签抗体特异性结合,特异性较强;比浊法测定复性蛋白比酶活为124.74 U/mg。本研究在大肠杆菌中表达了驴乳溶菌酶,并获得具有活性的复性蛋白,为驴乳溶菌酶的基础研究和应用奠定了基础。

To investigate the characteristics of donkey milk lysozyme,the donkey LYSC1 gene(DKLYSC1)was synthesised and cloned into the prokaryotic expression vector pET32 a(+).The expression and enzymatic activity of recombinant DKLYSC1 protein were detected by SDS-PAGE,western blot and nephelometry assay.Results showed that the recombinant DKLYSC1 protein was about 31 ku in molecular weight and mainly expressed in the form of inclusion bodies.The purified DKLYSC1 protein could specifically bind to the anti-His tag antibody detected by western blot analysis.The enzymatic activity of renaturated DKLYSC1 determined by nephelometry assay was 124.74 U/mg.In this study,donkey DKLYSC1 was successfully expressed in E.coli BL21(DE3)and an active protein was obtained after renaturation,laying the foundation for the basic research and application of DKLYSC1.