一种复合益生菌粉增强免疫力功能的动物研究

Effects of a probiotic blend in improving immunity in mice

目的通过细胞免疫、体液免疫、单核巨噬细胞吞噬功能、NK细胞活性测定试验检验由植物乳植杆菌HEAL9和副干酪乳酪杆菌8700:2配制而成的复合益生菌粉对小鼠免疫力功能的影响。方法将50只雄性BALB/c小鼠按体质量随机分为阴性对照组、阳性对照组以及益生菌粉低、中和高剂量组,每组10只,分别使用去离子水、转移因子口服液和相应浓度的益生菌粉进行灌胃给药,1次/d,连续30 d。实验过程中分别对小鼠进行迟发型变态反应试验、抗体生成细胞检测及血清溶血素生成试验。末次给药次日,对小鼠进行淋巴细胞转化的影响试验、碳廓清试验和腹腔巨噬细胞吞噬鸡红细胞试验。并委托细胞室进行靶细胞(YAC-1)传代,进行NK细胞活性试验。结果与阴性对照组相比,益生菌粉低、中、高剂量组小鼠脾淋巴细胞增殖能力、抗体积数及NK细胞活性均升高,益生菌粉高剂量组小鼠耳肿胀度、溶血空斑数、吞噬指数及吞噬百分率升高,差异均有统计学意义(P<0.05或P<0.01)。结论依照《保健食品功能评价方法(2020年版)(征求意见稿)》有助于增强免疫力功能检验方法及结果判定,判定该复合益生菌粉具有增强免疫力功能作用。

Objective To observe the effects of a co-supplement of probiotic blend powder consisting of Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2 on the immune function of mice.Methods A total of 50BALB/c mice were randomly divided into negative control group,positive control group,low-dose,medium-dose and highdose probiotic powder groups based on body weight(n=10).Each group of mice was feeding intragastrically transfer factor oral liquid,deionized water or probiotic powder,respectively,qd for 30 consecutive days.Delayed type hypersensitivity test,detection of antibody-forming cell and serum hemolysis test were conducted.Lymphocyte transformation test,carbon clearance test and chicken RBC phagocytosis test were conducted the day after the last administration.Passaging of target cells(YAC-1)was delegated to the cell department,and NK cell activity experiment was conducted.Results Comparing with the negative control group,the low-dose,medium-dose and high-dose probiotic powder groups had higher spleen lymphocyte proliferation,anti-volume value,and NK cell activity;ear swelling,hemolytic plaques,phagocytosis index and phagocytosis percentage increased in the high-dose probiotic powder group,with statistical differences(P<0.05 or P<0.01).Conclusion In accordance with the"Detection of Immune Function Enhancement"in Methods for Evaluation of Health Food Functions(2020 Edition)(Draft for Comment),it is determined that this probiotic powder has the function of enhancing immunity.