当归对阴虚哮喘小鼠气道黏液高分泌及TNF-α/NF-κB信号通路的影响

Effects of Radix Angelicae Sinensis on airway mucus hypersecretion and the TNF-α/NF-κB signaling pathway in Yin-deficiency asthmatic mice

目的:探讨当归对阴虚哮喘小鼠气道黏液高分泌及TNF-α/NF-κB信号通路的影响。方法:取KM小鼠随机分为空白对照组、模型对照组、氨溴索组、当归低、中、高剂量(2、4、8 g/kg)组(n=12),采用卵蛋白与甲状腺片复制阴虚哮喘模型,观测当归对小鼠哮喘症状、IgE、TNF-α以及肺组织Muc5ac与NF-κB表达的影响。结果:2、4、8 g/kg当归能明显缓解阴虚哮喘小鼠的哮喘症状,降低血清IgE与BALF中TNF-α水平,抑制肺组织Muc5ac与NF-κB的过度表达。结论:当归具有明显的平喘作用,抑制TNF-α/NF-κB信号通路而缓解气道黏液高分泌是其平喘的作用机制之一。

Objective:To investigate the therapeutic effects of Radix Angelicae Sinensis(RADA)on airway mucus hypersecretion and the tumor necrosis factor-α/nuclear factor-κB(TNF-α/NF-κB)signaling pathway in Yin-deficiency asthma mice.Methods:KM mice were randomly divided into control group,model group,ambroxol group and RADA low,medium and high dose(2,4 and 8 g/kg)group(n=12).Ovalbumin and the thyroid gland were used to replicate the model of Yin-deficiency asthma.Asthma symptoms in mice,immune globulin E(IgE),TNF-α,and the expressions of Mucin 5ac(Muc5ac)and NF-κB in lung tissue were observed under the intervention of RADA.Results:RADA at the doses of 2,4 and 8 g/kg could alleviate the asthma symptoms of Yin-deficiency asthma mice significantly,reduce the levels of IgE in serum and TNF-αin bronchoalveolar lavage fluid(BALF),and inhibite the overexpressions of Muc5ac and NF-κB in lung tissue.Conclusion:RADA has significant anti-asthmatic effect.One of its mechanisms is to inhibit TNF-α/NF-κB signaling pathway and to alleviate airway mucus hypersecretion.