斑马鱼发育毒性与致畸性相关实验研究进展

Advances in zebrafish experimental research on developmental toxicity and teratogenicity

自1994年Science、Nature等杂志发表专题评述,至2009年野生型AB品系斑马鱼全基因组测序完成,斑马鱼已经成为一种公认的新型模式生物。斑马鱼模式生物在发育毒性与致畸性研究中引人注目的优势是胚胎发育的透明性,基于高通量的检测体系,以及短时-高效-低费筛选,用于中药毒理和重大疾病中中药活性成分筛选,以及小分子化合物的大规模新药筛选研究。随着"3R原则"的推行与实施,斑马鱼可能发展成为发育毒性与致畸性试验哺乳动物替代模型。概括介绍了国内外斑马鱼发育毒性与致畸性研究最新进展。

Since the publication of special reviews and commentaries on zebrafish(Danio rerio)in Science,Nature,and other journals in 1994,and completion of the whole genome sequencing of wild type(AB strain)zebrafish in2009,the zebrafish has become a well-recognized new model organism.The remarkable advantages of zebrafish in the research and assessment of developmental toxicity and teratogenicity includes its transparency,high throughput,and rapid,high-efficiency,and low-cost screening.Zebrafish have been extensively used to screen the active components of traditional Chinese medicine for toxicological and pharmaceutical investigations,as well as for large-scale new drug screening of small molecule compounds.With the implementation of the"3 R principle(Reduction,Replacement,Refinement)",the zebrafish has been proposed as an alternative animal model for developmental toxicity and teratogenicity tests.The recent progress of developmental toxicity and teratogenicity assessments in zebrafish is reviewed in this report.