阿魏酸对高脂血症小鼠肝脂肪变性及肠道菌群的调节作用

Regulatory effects of ferulic acid on hepatic steatosis and intestinal flora in hyperlipidemic mice

目的探讨阿魏酸(ferulic acid)对高脂血症小鼠肝脂肪变性及肠道菌群的调节作用。方法将24只6周龄雄性Apo E-/-小鼠随机分为4组(n=6),即对照组、模型组、阿魏酸组[40 mg/(kg·d)]、辛伐他汀组[5 mg/(kg·d)];另取6只同龄C57BL/6小鼠为空白组。高脂喂养12周后,再给药处理12周,采集小鼠粪便进行肠道菌群检测,检测血脂水平,制作肝切片观察病理变化。结果与对照组相比,模型组小鼠体重、血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白(low-density lipoprotein cholesterol,LDL-C)明显升高(P<0.05),肝切片可见小鼠肝细胞质内充满脂滴,大部分肝细胞呈现脂肪变性,模型组厚壁菌门(Firmicutes)和韦荣球菌科(Erysipelotrichaceae)的细菌数量升高,拟杆菌门(Bacteroidetes)、瘤胃菌科(Ruminococcaceae)和Odoribacter数量减少;与模型组比较,阿魏酸组小鼠体重、血清TC、TG、LDL-C显著降低,肝脂肪变性明显减轻,厚壁菌门和韦荣球菌科细菌数量减少,拟杆菌门、瘤胃菌科和Odoribacter数量升高。结论阿魏酸可以改善小鼠的血脂异常,肝脂肪变性,调节肠道菌群失衡。

Objective To investigate the regulatory effects of ferulic acid on hepatic steatosis and intestinal flora in hyperlipidemic mice.Methods Twenty-four 6-week-old male Apo E-/-mice were randomly divided into the control,model,ferulic acid[40 mg/(kg·d)],and simvastatin groups[5 mg/(kg·d)];n=6 per group.Six 6-week-old male C57 BL/6 mice constituted the blank group.After 12 weeks of feeding a high-fat diet,the drugs were administered for another 12 weeks.The mouse feces were collected to detect the intestinal flora.Blood lipid levels were detected,and liver sections were prepared to observe the pathological changes.Results The body weight,serum total cholesterol(TC),triglycerides(TG),and low-density lipoprotein-cholesterol(LDL-C)levels were much higher in the model group than in the control group(P<0.05).Liver cells of the model group were full of lipid droplets,and most presented fatty degeneration.The amounts of Firmicutes and Erysipelotrichaceae in the model group were higher than those in the control group.Bacteroidetes,Ruminococcaceae and Odoribacter were decreased in the model group.Ferulic acid treatment significantly ameliorated the changes in body weight,serum TC,TG,and LDL-C(P<0.05).Ferulic acid upregulated Firmicutes and Erysipelotrichaceae and downregulated Bacteroidetes,Ruminococcaceae and Odoribacter.Ferulic acid also alleviated the liver injury in the high-fat diet-fed Apo E-/-mice.Conclusions Ferulic acid improves dyslipidemia and hepatic steatosis,and regulates the intestinal flora imbalance.