探讨活化蛋白C对大鼠心肌缺血再灌注损伤的保护作用及机制的影响

Investigation of the protective effect and mechanism of activated protein C on myocardial ischemia-reperfusion injury in rats

目的探讨活化蛋白C(APC)对大鼠心肌缺血再灌注损伤的保护作用与机制。方法纳入30只雄性SD大鼠随机分为假手术组(10只)、I/R组(10只)和APC处理组(10只)。结扎左冠状动脉前降支60 min,再灌注6 h制作心肌缺血再灌注模型;以ELISA和免疫组织化学法分别检测TNF-α、心肌组织MPO和ICM-1的表达水平;HE染色检测各组心肌病理学改变情况。结果与假手术组相比,I/R组的TNF-α和MPO、ICM-1的表达水平均明显增加(P<0.01);与I/R组比较,APC组的TNF-α、和MPO、ICM-1的表达水平均明显降低(P<0.01),而在光镜下观察炎性细胞浸润的情况,I/R组最为明显,APC组较IR组明显减轻。结论APC对心肌缺血再灌注损伤有保护作用,其机制可能与抑制炎性因子的表达,减轻组织炎性细胞浸润有关。

Objective To investigate the protective effect and mechanism of activated protein C(APC)on myocardial ischemia reperfusion injury in rats.Methods Thirty male SD rats were randomly divided into sham operation group(10),I/R group(10)and apc-treated group(10).The anterior descending branch of the left coronary artery was ligated for 60 min,and the myocardial ischemia reperfusion model was established 6 h after reperfusion.The expression levels of TNF-α,MPO and ICM-1 were detected by ELISA and immunohistochemistry.HE staining was used to detect the changes of myocardial pathology in each group.Results Compared with the sham group,the expression of TNF-α,MPO and ICAM-1 were increased significantly in the I/R group(P<0.01);but compared with the I/R group,the expression of TNF-α,MPO and ICAM-1 were obviously decreased in the APC group(P<0.01).Conclusions APC has a protective effect on myocardial I/R injury,in a mechanism that may involve inhibiting the expression of inflammatory factors and reducing the infiltration of inflammatory cells.