党参远志散微乳对阿尔茨海默病模型小鼠学习记忆障碍的改善作用

Dangshen Yuanzhi Powder microemulsion attenuates learning and memory impairment in Alzheimer’s disease model mice

目的研究党参远志散微乳对阿尔茨海默病(AD)模型小鼠脑组织中乙酰胆碱(Ach)和乙酰胆碱酯酶(AchE)水平及脑组织中AchE和乙酰胆碱转移酶(ChAT)蛋白表达的影响,运用胆碱能学说分析作用机制。方法小鼠随机分为对照组、模型组、生物相容性微乳组、脑复康组、党参远志散水提液组和党参远志散微乳组,每组15只。除对照组外,其余组均ip D-半乳糖(D-gal)和Na NO2制备AD小鼠模型,分别ig蒸馏水、生物相容性微乳、脑复康、党参远志散水提液和党参远志散微乳6周。采用水迷宫实验进行行为学考察;酶联免疫分析测定脑组织中Ach和Ach E水平;HE染色光镜观察海马CA1区和皮层的病理变化;免疫组织化学染色法检测海马CA1区和皮层的Ch AT、Ach E蛋白表达。结果与模型组比较,党参远志散微乳组小鼠学习记忆能力明显提高(P<0.05),脑组织中Ach水平明显升高(P<0.01),Ach E水平明显降低(P<0.01),海马CA1区和皮层的病理结构改变明显改善,Ch AT的蛋白阳性表达明显上升(P<0.01),Ach E蛋白阳性表达明显下降(P<0.01)。结论党参远志散微乳可通过上调小鼠海马CA1区和皮层中Ch AT表达和抑制Ach E表达而发挥抗AD作用。

Objective To observe the effects of Dangshen Yuanzhi Powder microemulsion on protein expression of AchE and ChAT,and the contents of Ach and AchE in brain tissue of Alzheimer’s disease model mice and analyze its mechanism based on cholinergic theory.Methods Mice were divided into control group,model group,BME group,Piracetam group,water extractant group and microemulsion extractant group,with 15 mice in each group.Alzheimer’s disease mice model was established by intraperitoneal injection with D-gal and NaNO2.Meanwhile,each group was given by gavage for six weeks with distilled water,BME,Piracetam tablets,water extractant and microemulsion extractant.Water maze was used to conduct behavior study.Contents of Ach and AchE in brain tissue were also measured.HE staining was used to observe pathological changes and IHC staining was used to detect expression of AchE and ChAT in hippocampal CA1 region and cerebral cortex of mice.Results Compared with the model group,learning and memory abilities of mice were significantly improved(P<0.05),content of Ach was significantly improved(P<0.01),content of AchE was significantly decreased(P<0.01),pathological changes of hippocampal CA1 region and cerebral cortex were improved,AChE expression was significantly downregulated(P<0.01),and ChAT expression was markedly upregulated(P<0.01)in microemulsion extractant group.Conclusion Dangshen Yuanzhi Powder microemulsion can control the progression of Alzheimer’s disease by upregulating ChAT expression and inhibiting AChE expression in hippocampal CA1 region and cerebral cortex of mice.