天南星及炮制品制天南星和胆南星水提物HPLC特征图谱比较研究

Comparative study on HPLC characteristic chromatogram of water extracts of Arisaematis Rhizoma and its processed products

目的建立天南星及炮制品制天南星和胆南星水提物的HPLC特征图谱,为鉴别天南星及炮制品制天南星和胆南星及其质量控制提供科学依据。方法采用HPLC法,色谱柱为Agilent C18(200 mm×4.6 mm,5μm),流动相为0.2%乙酸水溶液-0.2%乙酸乙腈溶液,梯度洗脱,对天南星及炮制品制天南星和胆南星的水提物进行研究,运用"中药色谱指纹图谱相似度评价系统(2012)"、SPSS 23.0和SIMCA 14.1软件进行相似度分析、聚类分析和主成分分析(PCA)。结果建立了天南星及炮制品制天南星和胆南星水提物的HPLC特征图谱,天南星、制天南星和胆南星各有19、20和13个共有峰,指认出F1(黄嘌呤)、F3(尿嘧啶)、F4(次黄嘌呤)、F5(尿苷)、F6(鸟苷)、F7(腺苷)、F14(夏佛塔苷)、F16(异夏佛塔苷)。天南星、制天南星和胆南星各自的相似度均在0.8以上且各聚为一类,天南星与炮制品制天南星和胆南星之间的相似度均在0.4以下,天南星的主成分有F16(异夏佛塔苷)、F14(夏佛塔苷)、F17、F15;制天南星有F16(异夏佛塔苷)和F1(黄嘌呤);胆南星有F3(尿嘧啶)、F2、F5(尿苷)、F1(黄嘌呤)。结论该方法可有效鉴别天南星及炮制品制天南星和胆南星,并为其质量控制提供一定的科学依据。

Objective To establish and analyze the HPLC characteristic chromatogram for water extracts of Arisaematis Rhizoma(AR) and its processed products, Arisaematis Rhizoma Preparatum(ARP) and Arisaema Cum Bile(ACB). The research provided reliable method and scientific basis for their quality control. Methods The separation was performed on an Agilent C18(200 mm × 4.6 mm, 5 μm) column with gradient elution of 0.2% acetic acid water and 0.2% acetic acid acetonitrile. The similarity was analyzed with software "Similarity Evaluation System for Chromatographic Fingerprint of TCMs(Version 2012)". The cluster analysis was performed by SPSS 23.0. The principal component analysis was performed by SIMCA 14.1. Results HPLC characteristic chromatogram for water extracts of AR, ARP, and ACB were established. There were 19, 20 and 13 common peaks in AR, ARP and ACB, respectively. A total of eight characteristic peaks were identified as F1(xanthine), F3(uracil), F4(hypoxanthine), F5(uridine), F6(guanosine), F7(adenosine), F14(schaftoside), and F16(isoschaftoside), respectively. The intragroup similarities of AR, ARP, and ACB were all above 0.8 and each was clustered into one type, and the intergroup similarities among AR and its processed products were all below 0.4. The main components of AR were F16(isoschaftoside), F14(schaftoside), F17 and F15. The main components of ARP were F16(isoschaftoside) and F1(xanthine). The main components of ACB were F3(uracil), F2, F5(uridine) and F1(xanthine). Conclusion The method can effectively identify AR, ARP and ACB, and provide a scientific basis for their quality control.