甘草总皂苷及水提物对肝损伤大鼠肠道菌群的影响

Total saponins of Glycyrrhiza inflata and its decoction on intestinal flora in rats with liver injury

目的探讨甘草总皂苷及水提物对肝损伤大鼠肠道菌群的影响。方法采用ip四氯化碳诱导大鼠慢性肝损伤模型,模型大鼠分为4组,分别ig给予甘草总皂苷和甘草水提物,并以联苯双酯作为阳性对照组,模型组ig生理盐水。同时设对照组,ig给予生理盐水。于给药结束后,测定各组大鼠血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平;收集大鼠粪便,通过16 S rRNA测序对各组大鼠肠道菌群的多样性进行分析。结果与对照组比较,模型组大鼠血清中ALT、AST水平显著升高(P<0.05、0.01);与模型组比较,各给药组大鼠血清中ALT、AST水平均显著降低(P<0.05、0.01);与模型组比较,联苯双酯组大鼠菌群结构未发生显著变化,而甘草总皂苷组及甘草水提物组均表现出不同的群落组成。并且相对于水提物组,总皂苷组显著提高了乳酸杆菌属和杆菌门S24-7科菌群的相对丰度。但甘草总皂苷组和甘草水提物组的大鼠肠道菌群与对照组大鼠肠道菌群之间仍存在差异,并没有完全恢复到正常状态。结论甘草总皂苷与甘草水提物均可以改善慢性肝损伤,并对肝损伤大鼠肠道菌群发挥不同的调控作用。

Objective To explore the effect of total saponins of Glycyrrhiza inflata and its decoction on intestinal flora in rats with liver injury.Methods Model of rats with liver injury was induced by intraperitoneal injection of carbon tetrachloride,which were randomly divided into four groups,including total saponins group(Sap group),water decoction group(Dec group),bifendate group(Bif group)and physiological saline group(NS group),then they were given drugs by oral admission.At the same time,a blank control was used(Control group),and the rats were given physiological saline by oral admission.Finally,the levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in the serum of each group of rats were measured;The rat feces were collected for 16 S rRNA sequencing of intestinal flora.Results Compared with the control group,the levels of ALT and AST in the serum of the model group were significantly increased(P<0.05,P<0.01).Compared with the model group,the levels of ALT and AST in the serum of the rats in each administration group were significantly reduced(P<0.05,P<0.01).Compared with the NS group,the intestinal flora in Bif group did not change significantly,while the Sap group and the Dec group showed different community composition.Total saponins significantly increased the relative abundance of Lactobacillus and Bacteroidales_S24-7 compared with water decoction.However,the intestinal flora of the Sap group and the Dec group were still different from the intestinal flora of the control group,and did not completely return to the normal state.Conclusion Total saponins of G.inflata and its decoction may play different roles in regulating the composition of intestinal flora in rats with liver injury,and then improve the pathological condition of liver injury.