不同软化方法对甘草质量的影响

Effects of different softening methods on quality of Glycyrrhizae Radix et Rhizoma

目的基于HPLC建立甘草指纹图谱及同时测定甘草中6个成分含量的分析方法,并考察4种软化方式(淋润、常压蒸润、70℃减压蒸润与85℃减压蒸润)对甘草质量的影响。方法采用紫外分光光度法,以甘草苷、甘草酸为对照品,测定甘草中总黄酮及总皂苷的含量;基于HPLC同时测定甘草中甘草苷、芒柄花苷、异甘草苷、甘草素、刺甘草查耳酮、甘草酸6个成分的含量,比较了不同软化方法处理后的甘草中指标成分含量的变化;利用中药色谱指纹图谱相似度评价系统对不同软化处理后的甘草样品进行相似度评价,并进行聚类分析。结果总黄酮、总皂苷的结果显示,未加工过的甘草中总黄酮与总皂苷的含量最高,淋润的甘草总黄酮与总皂苷含量偏低。常压蒸润、70℃减压蒸润和85℃减压蒸润这3种处理方法对甘草质量影响不大;含量测定结果显示,不同软化处理后,异甘草苷的含量均显著降低,而不同的软化处理组之间,所有成分含量差异不显著。指纹图谱相似性评价与聚类分析表明,3批甘草不同软化处理方法后的样品与未加工组分别聚为一类,而不同软化处理方法对于甘草整体化学成分组成无显著差异。结论建立的分析方法,能够快速、准确地同时测定甘草中的6种成分,而且尤其需要对软化过程中异甘草苷的含量进行监测。综合生产成本、生产效率及不同指标的质量评价结果,常压蒸润在生产过程中可行性最高。本研究为甘草软化的大生产提供了理论指导,有利于进一步规范甘草饮片的生产流程。

Objective A method was established to obtain fingerprint and determination of six components in Glycyrrhizae Radix et Rhizoma Pieces(GRRP)based on HPLC-PDA,and samples with four kinds of softening methods(showering moistening,steaming,70℃decompression steaming,85℃decompression steaming)were analyzed.Methods The content of total flavonoids and total saponins was determined by ultraviolet spectrophotometry with liquiritin and glycyrrhizic acid as reference materials.Simultaneous determination of six components of liquiritin,ononin,isoliquiritin,glycyrrhizin,echinatin,glycyrrhizic acid was performed based on HPLC.Changes of the components content in the samples which treated by different softening methods were compared.The similarity evaluation of samples with different softening methods was carried out by the chromatographic fingerprint similarity evaluation system of traditional Chinese medicine,and cluster analysis was also carried out.Results The results showed that the content of total flavonoids and total saponins in untreated samples was the highest,and the content of total flavonoids and total saponins in samples treated by showering moistening was the lowest.The three treatment methods of atmospheric pressure steaming,steaming decompression at 70℃and steaming decompression at 85℃had little effect on the samples.The content determination showed that the content of isoliquiritin was decreased significantly after softening treatment.The difference among the different softening treatment groups was not significant.The samples with different softening methods of the three batches of samples were grouped together with their raw products.Different softening methods had no significant difference in the composition of the medicinal herbs.Conclusion The established method can quickly and accurately determine the six components,and in particular,the content of isoglycyrrhizin should be monitored.Combining production efficiency,production cost and quality evaluation,steaming is the most feasible in the production process.This study provided theoretical guidance for the large-scale production of softening,which was conducive to further standardizing the production process of GRRP.