抗人白细胞单克隆抗体1C34-5与正常及肿瘤组织或细胞的反应性

The reactivity of a monoclonal anti-human leucocyte antibody 1C34-5 with human normal and malignant tissues or cells

用免疫组化方法及EUSA测检研究抗人白细胞单克隆抗体1C34-5与正常及肿瘤细胞组织的反应性,发现1C34-5仅与正常的白细胞和淋巴造血组织来源的肿瘤细胞反应。在所观察的1例毛细胞白血病、10例恶性淋巴病,2例何杰金氏病的肿瘤组织均与1C34-5呈不同程度的阳性反应,而31例各种不同类型非淋巴造血组织来源的肿瘤均为阴性反应。浸润到肿瘤细胞间或邻近部位的淋巴样细胞也为阳性反应。此抗体在辅助鉴别形态学难以区分的小圆细胞癌和淋巴瘤的病理诊断中,将有一定意义。此外也为观察判断转移癌细胞分布及浸润程度,以及宿主抗肿瘤的免疫反应提供了一种新手段。

The reactivity of a monoclonal anti-human leucocyte antibody,designated 1C34-5,with.various human normal and malignant tissues were tested by means of ELLSA as well as immunohistochemical staining.It was found that this antibody reacted only with normal leucocytes and malignant heamopoietic tissues so far tested.Pathological samples from 1 case of hairy cell leukemia,10 cases of malignant lymphoma and 2 cases of Hodgkin’s disease showed positive staining.In contrast,all 31 ofses of non-heamop-oietic malignant tumours failed to react with this antibody.The lymphoid cells infiltrating into malignant tissues or in the normal tissues adjacent to tumouts also showed positive reaction.Therefor,this antibody has a potential as a diagnostic reagent to differentiate small-cell anaplastic carcinomas from malignant lymphoma.