An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2

Dear Editor,Severe acute respiratory syndrome coronavirus-2(SARS-Co V-2)is a novel coronavirus that causes the outbreak of coronavirus disease 2019(COVID-19)(Li et al.,2020a).Viral nucleic acid testing is the standard method for the laboratory diagnosis of COVID-19(Wu et al.,2020a;Zhu et al.,2020).Currently,a variety of qPCR-based detection kits are used for laboratory-based detection and confirmation of SARS-CoV-2 infection(Corman et al.,2020;Hussein et al.,2020;Ruhan et al.,2020;Veyer et al.,2020).Conventional qPCR involves virus inactivation,nucleic acid extraction,and qPCR amplification procedures.Therefore,the process is complicated,which usually takes longer than 2 h,and requires biosafety laboratories and professional staff.Thus,qPCR is not suitable for use in field or medical units.