摘要
West Nile virus(WNV)is an important neurotropic flavivirus that is widely distributed globally.WNV strain XJ11129 was first isolated in Xinjiang,China,and its genetic and biological characteristics remain largely unknown.In this study,phylogenetic and sequence analyses revealed that XJ11129 belongs to lineage 1 a and shares high genetic identity with the highly pathogenic strain NY99.Then,the full-length genomic c DNA of XJ11129 was amplified and assembled using a modified Gibson assembly(GA)method.The virus(named r XJ11129)was successfully rescued in days following this method.Compared with other wild-type WNV isolates,r XJ11129 exhibited virulence indistinguishable from that of the NY99 strain in vivo.In summary,the genomic and virulence phenotypes of r XJ11129 were characterized in vivo and in vitro,and these data will improve the understanding of the spread and pathogenesis of this reemerging virus.
基金
supported by the National Key Research and Development Project of China(2016YFD0500304)
the National Science and Technology Major Project of China(2018ZX09711003)
the National Natural Science Foundation of China(81621005 and 31770190)
supported by the National Science Fund for Distinguished Young Scholars(81925025)
the Innovative Research Group(81621005)from the NSFC
the Innovation Fund for Medical Sciences(2019-I2M-5–049)from the Chinese Academy of Medical Sciences
