木犀草素提高机体耐缺氧能力研究

ANTI-HYPOXIA EFFECTS OF LUTEOLIN IN MICE

目的探索木犀草素的抗缺氧作用,为抗缺氧新药物的研制提供思路。方法将40只昆明(KM)雄性小鼠分为4组,每组10只,连续灌胃(0.5%羧甲基纤维素钠溶液、25、50和100mg/kg木犀草素溶液)15d,进行常压密闭缺氧实验,记录小鼠存活时间(survival time,ST)、检测小鼠血清以及心、肝等组织的谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)和总超氧化物歧化酶(total-superoxide dismutase,T-SOD)活力,总抗氧化能力(total antioxidant capacity,T-AOC),丙二醛(malondialdehyde,MDA)含量,观察木犀草素对常压缺氧小鼠的保护作用。结果与对照组相比,木犀草素中、高剂量灌胃给预组小鼠在常压密闭缺氧条件下的常压缺氧密闭时间(the survival time,ST)、标准耐缺氧时间(standard hypoxia tolerance time,STT)和校准后的标准耐缺氧时间(adjusted standard hypoxia tolerance time,ASTT)均显著延长(P<0.05),木犀草素高剂量组小鼠血清中GSH-PX和T-SOD活力显著提高(P<0.05)、肝组织中MDA含量显著下降(P<0.05)、心脏组织T-SOD活力显著提高(P<0.05)。结论中、高剂量的木犀草素可以显著延长小鼠的缺氧存活时间,具有抗缺氧作用。

Objective To explore the anti-hypoxia effect of luteolin,so as to provide data for the development of new drugs.Methods Forty KM male mice were divided into four groups,and intragastrically administered 0.5%sodium carboxymethyl cellulose solution,25mg/kg luteolin solution,50 mg/kg luteolin solution,100 mg/kg luteolin solution,respectively for 15 days.To observe the protective effect of luteolin on normobaric hypoxia,the mice were subjected to hypoxia condition under normal pressure.The survival time(ST)was recorded.The activities of glutathione peroxidase(GSH-Px)and total-superoxide dismutase(T-SOD),the ability of total antioxidant capacity(T-AOC)and the content of malondialdehyde(MDA)in serum,heart and liver were detected.Results Compared with the control group,the survival time,standard hypoxia tolerance time(STT)and calibrated standard hypoxia tolerance time(ASTT)of the mice in moderate and high luteolin doses groups were significantly prolonged(P<0.05).The activities of serum GSH-Px and T-SOD in mice in high luteolin dose group were significantly increased(P<0.05).The content of MDA in the liver of mice in high luteolin dose group were significantly decreased(P<0.05).The activity of T-SOD in the myocardial tissue of mice in high luteolin dose group were significantly increased(P<0.05).Conclusion Moderate and high doses of luteolin can significantly prolong the survival time of mice under hypoxia condition.