基于药食同源的复方制剂抗疲劳效果评价

EVALUATION OF ANTI-FATIGUE ACTION OF COMPOUND PREPARATIONS BASED ON THE PRINCIPLE OF MEDICINE AND FOOD HOMOLOGY

目的以黄芪、当归、枸杞子、人参、桃仁等为主要成分,设计3种不同配伍的功能制剂,评价其抗疲劳效果,并探讨相关生化机制。方法(1)实验1.三种复方制剂抗疲劳效果的比较研究。48只5~6周龄雄性BALB/c小鼠随机分为运动对照组(EC组)、制剂1组(F_(1)组)、制剂2组(F_(2)组)和制剂3组(F_(3)组),每组12只小鼠。EC组小鼠灌胃纯净水,F_(1)、F_(2)和F_(3)组灌胃不同组方的汤剂20ml/kg·BW,每日1次,干预28d。末次灌胃后30min进行负重力竭游泳实验,比较不同制剂干预对小鼠力竭游泳时间的影响。(2)实验2.复方制剂的抗疲劳机制探讨。30只5~6周龄雄性BALB/c小鼠随机分为安静对照组(NC组)、运动对照组(EC组)和中药方剂组(FZ组),每组10只小鼠。NC组和EC组灌胃纯净水,FZ组灌胃实验1中筛选的复方制剂20ml/kg·BW,每日1次,干预28d。末次灌胃后30min,NC组即刻、EC组和FZ组完成非负重60min游泳实验后处死取材。用生化试剂盒检测小鼠血清中乳酸(BLA)、尿素氮(BUN)的含量及乳酸脱氢酶(LDH)活性;肝组织中肝糖原、丙二醛(MDA)的含量及超氧化物歧化酶(SOD)活性;肌肉组织中肌糖原、MDA的含量以及Na^(+)-K^(+)-ATP酶和Ca^(2+)-Mg^(2+)-ATP酶的活性。结果(1)在三种复方制剂抗疲劳效果的比较研究中观察到,与EC组相比,F_(2)组小鼠负重力竭游泳时间显著延长(P<0.01)。(2)在复方制剂的抗疲劳机制探讨中观察到,①与NC组相比,EC组和FZ组小鼠血清中BUN和BLA含量显著升高(P<0.05),LDH活性显著降低(P<0.05);与EC组相比,FZ组小鼠血清中BUN和BLA含量显著降低(P<0.05),LDH活性显著升高(P<0.05)。②与NC组相比,EC组和FZ组小鼠肝组织中肝糖原含量显著降低(P<0.05),MDA含量显著升高(P<0.01;P<0.05);与EC组相比,FZ组小鼠肝组织MDA含量显著降低(P<0.05)。③与NC组相比,EC组和FZ组小鼠肌肉组织中肌糖原含量显著降低(P<0.05),MDA含量显著升高(P<0.01;P<0.05),Na^(+)-K^(+)-ATP酶和Ca^(2+)-Mg^(2+)-ATP酶活性显著降低(P<0.05);与EC组相比,FZ组小鼠肌肉组织中肌糖原含量显著升高(P<0.05),MDA含量显著降低(P<0.05)。结论以黄芪、当归、枸杞子为主进行配伍的复方制剂具有显著抗疲劳作用;机制可能涉及增加糖原储备以及提高抗氧化能力。

Objective Based on the homology of medicine and food,three different compatible compound preparations were designed with astragalus membranaceus,angelica sinensis,wolfberry fruit,ginseng and peach kerne as the main components and their anti-fatigue effects were evaluated.Methods(1)ExperimentⅠ:Comparative study on anti-fatigue effects of three compound preparations.Forty-eight male BALB/c mice aged 5-6 weeks were randomly divided into exercise control group(EC group),preparation group 1(F_(1)group),preparation group 2(F_(2)group)and preparation group 3(F_(3)group),with 12 mice in each group.Mice in EC group were gavaged with purified water,and mice in F_(1),F_(2)and F_(3)groups were gavaged with 20 ml/kg·BW of decoctions from different preparations,once a day,for 28 days.The loaded exhaustive swimming experiment was carried out 30 minutes after the last gavage,and the effects of different preparations on the time of exhaustive swimming were compared.(2)ExperimentⅡ:study on anti-fatigue mechanism of compound preparation.Thirty male BALB/c mice aged 5-6 weeks were randomly divided into normal control group(NC group),exercise control group(EC group)and traditional Chinese medicine formula group(FZ group),with 10 mice in each group.NC group and EC group were given purified water by gavage,and FZ group was given 20 ml/kg·BW of functional preparation selected in ExperimentⅠby gavage,once a day,for 28 days.At 30min after the last gavage,the NC group was sacrificed immediately,and the EC group and FZ group were sacrificed after 60min unloaded swimming experiment.The contents of blood lactic acid(BLA),urea nitrogen(BUN)and the activity of lactate dehydrogenase(LDH),the contents of hepatic glycogen,malondialdehyde(MDA)and the activity of superoxide dismutase(SOD)in liver tissue,the contents of muscle glycogen,MDA and the activities of Na^(+)-K^(+)-ATPase and Ca^(2+)-Mg^(2+)-ATPase in muscle tissue were detected by biochemical kits.Results(1)In the comparative study of the anti-fatigue effects of the three compound preparations,it was observed that the loaded exhaustive swimming time of F_(2)group mice was significantly longer than that of EC group(P<0.01).(2)In the study of anti-fatigue mechanism of compound preparation,following results were obtained.(1)Compared with the NC group,the contents of BUN and BLA in the serum of EC group and FZ group were significantly increased(P<0.05),and the activity of LDH was significantly decreased(P<0.05).Compared with the EC group,the contents of BUN and BLA in the serum of the FZ group decreased significantly(P<0.05),and the activity of LDH increased significantly(P<0.05).(2)Compared with the NC group,the content of liver glycogen in the EC group and FZ group decreased significantly(P<0.05),and the content of MDA increased significantly(P<0.01;P<0.05).Compared with the EC group,the content of MDA in the liver of the FZ group decreased significantly(P<0.05).(3)Compared with the NC group,the content of muscle glycogen in the EC group and FZ group decreased significantly(P<0.05),the content of MDA increased significantly(P<0.01),and the activities of Na^(+)-K^(+)-ATPase and Ca^(2+)-Mg^(2+)-ATPase decreased significantly(P<0.05).Compared with the EC group,the content of muscle glycogen in the FZ group increased significantly(P<0.05),and the content of MDA decreased significantly(P<0.05).Conclusion The preparation containing radix astragali,angelica sinensis and fructus lycii has significant anti-fatigue action.The mechanism may involve increasing glycogen reserve and enhancing antioxidant capacity.