乌腺金丝桃提取物对降低脂质生成与改善肥胖大鼠肝脂质沉积的作用

ROLES OF HYPERICUM ATTENUATUM CHOISY EXTRACTS IN ALLEVIATING HEPATIC LIPID ACCUMULATION AND DE NOVO LIPOGENESIS IN OBESE RATS

目的探讨乌腺金丝桃醇提物(HACE)对抑制大鼠肥胖和恢复肝功能状态的作用。方法本研究选用共40只SD雄性大鼠根据正常饮食和高脂饮食分为5组,每组共8只。其中高脂饮食诱导下的肥胖动物组分别采用蒸馏水、乌腺金丝桃提取物100、200 mg/kg和二甲双胍(Met)100 mg/kg进行灌胃干预。喂养8w后禁食过夜16h后称重并测量空腹血糖,麻醉解剖取血进行生化指标检测,收集肝脏、附睾脂肪、肾周脂肪和腹部脂肪,称重冻存。肝脏切去一叶置于甲醛中,进行H&E组织学染色分析。结果与正常组比较,模型组大鼠生化指标中血脂水平和肝损伤水平显著升高(P<0.01)。而与模型组大鼠相比,HACE组和Met组中的TC、TG、LDL-C、AST和ALT水平显著降低(P<0.01)。并且HACE组和Met组的Leptin水平显著降低(P<0.01),Adiponectin水平显著恢复(P<0.01)。此外,研究表明,与高脂饮食大鼠组相比较,HACE组和Met组的大鼠体重、肝重、脂肪含量的增长均得到有效抑制(P<0.01),肝脏内TGs、TC水平降低(P<0.01)。经H&E染色后的组织学观察结果发现,补充HACE后,肥胖导致的肝脏脂质沉积和囊泡增多现象得到了显著改善,表明HACE可有效改善血脂异常和肝功能损伤,抑制脂肪的形成。RT-PCR研究进一步的表明,HACE是通过降低脂质生成相关的转录因子及其下游基因的表达,进而抑制大鼠肥胖的发生。结论HACE可有效抑制肝脏脂质生成作用和甘油三酯的产生,保护肥胖大鼠肝功能的损伤,改善代谢紊乱及调控相关转录因子的转录途径,进而抑制肝脏脂肪变性。

Objective To explore the role of HACE in the prevention of obesity and restoration of hepatic functional capacity in rats.Methods A total of 40 SD male rats were divided into one normal diet group and four high fat diet(HFD)groups with eight rats in each group.HFD-induced obese rats were subjected to oral gavage intervention with HACE 100 and200 mg/kg and metformin 100 mg/kg,respectively.After 8 weeks of feeding,the rats were fasted overnight prior to blood glucose measurement.Blood was taken and the liver,epididymal fat,perirenal fat and abdominal fat were harvested for biochemical analysis.A lobe of the liver was dissected and placed in formaldehyde for H&E histological staining analysis.Results The hyperlipidemia and hepatic injury were significantly increased in the HFD group compared to the NCD group(P<0.01).In contrast,the levels of TC,TG,LDL-C,AST and ALT were significantly lower in the HACE and Met groups than in the HFD groups(P<0.01).In addition,the leptin index was markedly lower(P<0.01)and the restored adiponectin index was obviously observed(P<0.01).The gain of body weight,liver weight and adipose content of rats in the HACE and Met groups were effectively inhibited and the levels of hepatic TGs and TC were reduced compared with the HFD group.Furthermore,histological analysis revealed that obesity-induced hepatic lipid accumulation and vesicular increase were improved after HACE supplementation.The RT-PCR study showed that HACE inhibited the development of obesity in rats by reducing the expressions of lipogenesis-related transcription factors and their downstream genes.Conclusion HACE supplementation can effectively inhibit hepatic lipogenesis and triglyceride production,protect rats against liver functional injury,improve metabolic disorders and regulate the transcriptional pathways of related transcription factors,and thus suppress hepatic steatosis.