桂东南地区宫颈鳞状细胞癌组织O-6-甲基鸟嘌呤-DNA甲基转移酶的表达及临床意义

Expression and clinical significance of MGMT in cervical squamous cell carcinoma tissues in southeast Guangxi

目的基于临床病理样本分析桂东南地区宫颈鳞状细胞癌患者中O-6-甲基鸟嘌呤-DNA甲基转移酶(O-6-methylguanine-DNA methyltransferase,MGMT)蛋白的表达及其与临床病理特征的关系。方法收集桂东南地区人群的非癌宫颈、宫颈上皮内瘤变及宫颈鳞状细胞癌标本各200例,利用免疫组织化学染色检测MGMT蛋白的表达,分析其与临床病理特征的关系。利用bibliometrix对来自Web of Science Core Collection中62篇主题为MGMT和宫颈癌的文章进行文献计量学分析。结果在炎性宫颈、宫颈上皮内瘤变、宫颈鳞状细胞癌中,MGMT的表达逐渐降低(P<0.05),低分化癌中MGMT蛋白的表达较中-高分化癌降低。伴淋巴结转移的宫颈鳞状细胞癌中MGMT表达较无淋巴结转移组降低(P<0.05),三年随访结果发现死亡组MGMT蛋白阳性表达率低于存活组(P<0.05)。文献计量学结果表明2001—2019年内DNA甲基化、癌症、细胞凋亡等话题研究热度逐渐升高。结论MGMT的低表达与宫颈鳞状细胞癌的发生、组织学分级、淋巴结转移及患者预后密切相关,其潜在分子机制仍需进一步探究。

Objective To analyze the expression of O-6-methylguanine-DNA methyltransferase(MGMT)protein in cervical squamous cell carcinoma in southeastern Guangxi based on clinicopathological samples and its relationship with clinicopathological features.Methods A total of 200 cases of non-cancerous cervix,200 cases of cervical intraepithelial neoplasia and 200 cases of cervical squamous cell carcinoma were collected from southeast Guangxi.The expression of MGMT protein was detected by immunohistochemical staining,and the relationship between MGMT protein expression and clinicopathological features was analyzed.bibliometrix was used to conduct a bibliometric analysis of 62 articles from the Web of Science Core Collection on the topic of MGMT and cervical cancer.Results MGMT expression gradually decreased in inflammatory cervix,cervical intraepithelial neoplasia and cervical squamous cell carcinoma(P<0.05),and MGMT protein expression in poorly differentiated carcinoma was significantly lower than that in moderately and well differentiated carcinoma.The expression of MGMT in cervical squamous cell carcinoma with lymph node metastasis was lower than that in cervical squamous cell carcinoma without lymph node metastasis(P<0.05).The positive expression rate of MGMT protein in the death group was significantly lower than that in the survival group(P<0.05).The results of bibliometrics showed that the research on DNA methylation,cancer,apoptosis,and other topics gradually increased from 2001 to 2019.Conclusion The low expression of MGMT is closely related to the occurrence,histological grade,lymph node metastasis and prognosis of cervical squamous cell carcinoma,and its underlying molecular mechanism still needs to be further explored.