谷胱甘肽过氧化物酶1(GPX1)在肝癌组织中的表达及临床意义

Expression and clinical significance of glutathione peroxidase 1(Gpx1)in hepatocellular carcinoma

目的观察肝癌组织中检测谷胱甘肽过氧化物酶1(glutathione peroxidase 1,GPX1)异常表达水平,探讨其临床意义。方法选择河南省开封市传染病医院病理科2018年7月—2019年6月存档的77例原发性肝癌患者组织标本蜡块作为观察组,选择同期50例正常肝组织标本作为对照组。采用免疫组织化学(immunohistochemistry,IHC)法检测肝癌组织的GPX1表达水平,比较正常肝组织及肝癌组织、不同分期肝癌分组(Ⅰ期34例、Ⅱ期21例、Ⅲ期19例、Ⅳ期3例)、不同性别(男39例、女38例)肝癌分组GPX1表达水平,并分析GPX1表达与肝癌预后相关性(平均总体生存时间(overall survival,OS)以及无复发生存时间(recurrence-free survival,RFS)。结果观察组(肝癌组织)GPX1表达水平明显高于对照组(正常肝组织),差异有统计学意义(P<0.05)。肝癌组织Ⅱ期GPX1表达水平明显高于Ⅲ期、Ⅰ期、Ⅳ期,差异有统计学意义(P<0.05),而Ⅳ期GPX1表达水平明显低于Ⅲ期、Ⅰ期,差异有统计学意义(P<0.05)。肝癌组织男性患者GPX1表达水平明显高于女性患者,差异有统计学意义(P<0.05)。肝癌组织GPX1高表达患者OS、DFS明显低于低表达患者,差异有统计学意义(P<0.05)。结论GPX1高表达水平主要分布于男性、肝癌组织以及Ⅱ期分化程度患者中,GPX1水平表达可以诊断及鉴别肝癌组织分化程度,预测患者预后结局,指导临床规范治疗。

Objective To confirm the abnormal expression of glutathione peroxidase 1(GPX1)in hepatocellular carcinoma(HCC)tissues and determine its clinical significance.Methods The paraffin blocks of 77 primary liver cancer cases archived in the Department of Pathology(Infectious Diseases Hospital of Kaifeng,Henan,China)from July 2018 to June 2019 were designated as the observation group,and 50 normal liver tissue samples obtained during the same time period were designated as the control group.Immunohistochemistry(IHC)was used to detect the level of GPX1 expression in liver cancer tissues.The levels of GPX1 expression in normal liver,liver cancer subgroups based on stage(stageⅠ,n=34;stageⅡ,n=21;stageⅢ,n=19;and stageⅣ,n=3),and liver cancer subgroups based on gender(male,n=39;and female,n=38)were compared,and the correlation between GPX1 expression and liver cancer prognosis was analyzed(mean overall survival[OS]and recurrence-free survival[RFS]).Results The level of Gpx1 expression in the observation group(liver cancer tissue)was significantly higher than the control group(normal liver tissue;P<0.05).The level of Gpx1 expression in the stageⅡsubgroup was significantly higher than the stage 1,Ⅲ,andⅣsubgroups(P<0.05),while the level of Gpx1 in the stageⅣsubgroup was significantly lower than stagesⅠandⅢ(P<0.05).The level of Gpx1 expression in the male subgroup was significantly higher than the female subgroup(P<0.05).OS and disease-free survival were significantly lower in patients with high Gpx1 expression than patients with low Gpx1 expression(P<0.05).Conclusion The high level of Gpx1 expression was mainly distributed in males,liver cancer tissues,and patients with stageⅡdifferentiation.The level of Gpx1 expression can be used to diagnose patients with HCC,differentiate the degree of liver cancer tissues,determine patient prognosis,and guide the clinical standard treatment.