Nafion-碳纳米管修饰电极电化学发光分析法测定卡马西平

Electrochemiluminescence Determination of Carbamazepine Based on the Nafion-Carbon Nanotube Modified Electrode

建立了一种利用修饰电极检测卡马西平含量的电化学发光新方法。首先通过滴涂与离子交换法制备了Nafion-碳纳米管(CNTs)/Ru(bpy)32+玻碳修饰电极,并利用循环伏安法与电化学发光信号-电势图谱对其进行了初步表征,同时初步探讨了该方法检测卡马西平含量的可能原理;再基于中性介质中,少量卡马西平能显著增敏Ru(bpy)32+的电化学发光信号,利用增敏的电化学发光强度对卡马西平进行分析检测。在优化的实验条件下,卡马西平浓度在3.0×10-9~1.0×10-7 mol/L浓度范围内与增敏的电化学发光信号呈现良好的线性相关,检出限与相对标准偏差分别为1.5×10-9 mol/L及1.9%(c=1.0×10-8 mol/L,n=11)。应用该方法测定片剂卡马西平含量,平均回收率为102.3%,结果较为满意,有望应用于临床检验中卡马西平含量的准确分析。

A novel electrochemiluminescence(ECL)method with the modified electrode for detecting carbamazepine was established.Firstly,a Nafion-Carbon nanotubes(CNTs)/Ru(bpy)32+modified electrode(Nafion-CNT/Ru(bpy)32+/GCE)was prepared by coating and ion exchange strategy,and was primarily characterized by cyclic voltammetric method and ECL-potential spectrograms.Moreover,this work preliminarily discusses the possible principle for the determination of carbamazepine.In the neutral medium,it is found that a small amount of carbamazepine could significantly heighten the ECL signal of Ru(bpy)32+.By enhancing the ECL signal,the content of carbamazepine can be quantitatively determined.Under the optimal conditions,a good linear relationship between the enhancing ECL signal and carbamazepine concentration from 3.0×10-9 to 1.0×10-7 mol/L is obtained.The detection limit and relative standard deviation(RSD)are 1.5×10-9 mol/L and 1.9%(c=1.0×10-8 mol/L,n=11),respectively.This method is applied to the detection of carbamazepine in tablets with an average recovery of 102.3%.The results are more satisfactory and the proposed method can be applied to accurate analysis of carbamazepine content in clinical examination.