超高效液相色谱-三重四极杆质谱联用结合固相甲基化技术测定不同生长环境人参中寡糖分布

Determination of Oligosaccharides in Ginseng from Different Growth Environments by Ultra Performance Liquid Chromatography Triple Quadrupole Tandem Mass Spectrometry Combined with Solid Phase Methylation

以NaOH为填料制备固相甲基化柱,以CH3I为甲基化试剂,利用超高效液相色谱-三重四极杆质谱联用技术(UPLC-QQQ-MS)检测寡糖甲基化产物。在前期研究工作基础上,进一步优化固相甲基化条件。与寡糖对照品相对保留时间和质谱碎片信息比对,在人参中共检测到6种还原寡糖(麦芽糖、麦芽三糖、麦芽四糖、麦芽五糖、麦芽六糖、麦芽七糖)和3种非还原寡糖(蔗糖、蔗果三糖、蔗果四糖)。该方法可应用于同时测定上述人参中9种寡糖的含量。采用UPLC-QQQ-MS结合固相甲基化技术可对人参寡糖进行高效衍生和定量分析,为探讨生长环境对人参中寡糖分布的影响提供了有效方法。

The solid phase methylation column was packed with NaOH and CH3I was used as the methylation reagent.The methylated oligosaccharides products were characterized by ultra performance liquid chromatography triple quadrupole tandem mass spectrometry(UPLC-QQQ-MS).The solid phase methylation conditions were further optimized based on previous work.Six reducing oligosaccharides(maltose,maltotriose,maltotetraose,maltopentaose,maltohexaose and maltoheptaose)and three non-reducing oligosaccharides(sucrose,kestose and nystose)were detected in ginseng.This method could be applied in the quantitation of nine oligosaccharides in ginseng,simultaneously.UPLC-QQQ-MS combined with solid phase methylation could be used to efficiently derive and quantify oligosaccharides in ginseng.Therefore,an effective method is devdeloped to investigate the influence of growth environment on the distribution of oligosaccharides in ginseng.