734例HIV抗体初筛阳性标本的复检和确证结果分析

Analysis on retest and confirmation results of 734 HIV antibody screening positive specimens

目的分析威海市HIV初筛、复检和确证结果的关系,了解全市HIV检测水平,为威海市艾滋病防治提出针对性策略。方法采用第4代酶联免疫吸附试验(ELISA)及胶体硒试验,对威海市2015~2018年各筛查实验室送检的初筛阳性标本进行复检,使用免疫印迹试验(WB)对复检阳性标本进行确证试验。结果734例筛查阳性标本中复检阳性502例,确证阳性460例,确证阴性11例,不确定31例。不同来源(医疗、疾控、采供血机构)初筛标本的复检符合率差异有统计学意义(P<0.01)。确证阳性标本中男女比例为16.7∶1。确证阳性标本WB各条带中gp160、gp120出现率最高均为100.00%,p55出现率最低为44.35%。不确定标本的WB带型有8种。结论2015~2018年威海市各检测机构HIV复检符合率较低。

Objective To analyze the relationship of screening test,retest and confirmation results of HIV positive specimens in Weihai city,2015-2018,so as to understand the HIV detection level and propose targeted strategies for HIV/AIDS prevention and control in Weihai city.Methods The fourth-generation Enzyme-linked immunosorbent assay(ELISA)and the electro selenium screening were used for HIV retest of positive screening samples sent by screening laboratories in Weihai City from 2015 to 2018,and the western blotting(WB)was used for confirmatory testing.Results Among the 734 HIV screening positive cases,502 cases were retested positive,460 cases were confirmed positive,11 cases were confirmed negative,and 31 cases were uncertain.The difference in the coincidence rate of the retests of the initial screening specimens from different sources(medical institution,CDC,blood center)of screening laboratories was statistically significant(P<0.01).The sex ratio(male to female)was 16.7:1.The appearance rates of the strip of gp160 and gp120 from WB bands of confirmed positive specimens were the highest at 100%,and the p55 was 44.35%.There are 8 types of WB bands of uncertain cases.Conclusion The retest compliance rate of HIV testing in Weihai city from 2015 to 2018 is relatively low.