金莲花酚酸类成分的大鼠肝微粒体生物转化研究

Rat liver microsomal metabolism of phenolic acids from the flowers of Trollius chinensis

目的研究金莲花中酚酸类化合物在大鼠肝微粒体内的转化特征。方法建立体外大鼠肝微粒体转化模型,模拟金莲花中原金莲酸、金莲花苷、藜芦酸在大鼠肝脏内的代谢过程,采用液相色谱质谱联用(LC-MS)法对代谢成分进行检测和分析。结果在大鼠肝微粒体的作用下,原金莲酸转化为1个产物,分子式为C19H23O10,推测为原金莲酸的葡萄糖醛酸化反应产物;金莲花苷转化为2个产物,分别为C25H36O14和C18H24O9,推测分别为增加1糖基和脱甲基生成的转化产物;藜芦酸转化为1个产物,分子式为C8H8O4,推测2种结构,各为脱掉1个甲基的产物。结论金莲花酚酸类化合物在大鼠肝微粒体中主要发生脱甲基化、糖苷化、葡萄糖醛酸化反应,为明确金莲花酚酸类成分的体内转化规律和代谢特征提供依据。

Objective To explore the metabolic characteristics of phenolic acids from the flowers of Trollius chinensis in rat liver microsomes.Methods The rat liver microsomal transformation model was established in vitro to simulate the metabolic process of proglobeflowery acid,trollioside,and veratric acid in rat liver.LC-MS method was employed to detect and analyze the metabolic components.Results Under the action of rat liver microsomes,proglobeflowery acid was transformed into one product,with the molecular formula of C19H23O10,which was supposed to be the glucuronization reaction product of proglobeflowery acid.Trollioside was transformed into two products,C25H36O14 and C18H24O9,which were supposed to be the transformation products after adding one glycosyl group and removing one methyl group,respectively.Veratric acid was transformed into one product,with the molecular formula of C8H8O4,which was supposed to have two structures.It is supposed to be the product of removing one methyl group.Conclusion Glucuronidation,demethylation and glycosylation are mainly involved in the rat liver microsomal of transformation of phenolic acids from the flowers of Trollius chinensis.It provides a basis for the characterization of metabolic profiling of the phenolic components from the flowers of Trollius chinensis..