注射用益气复脉(冻干)不同给药途径对小鼠心肌梗死的改善作用研究

Effects of Yiqi Fumai Lyophilized Injection of different routes of administration against acute myocardial infarction mice

目的比较注射用益气复脉(冻干)(YQFM)尾iv与ig两种给药途径对心肌梗死小鼠的改善作用。方法采用冠状动脉左前降支结扎诱导小鼠急性心肌梗死模型,饲养1周得到慢性心肌梗死模型。分别通过尾iv与ig给药两种途径给予临床等效剂量的YQFM(0.867 g/kg),通过测定不同给药时间后血清中乳酸脱氢酶(LDH)和肌酸激酶(CK)水平,比较两种给药途径起效时间。进一步开展心脏超声、超氧化物歧化酶(SOD)测定以及心脏HE染色实验,评价两种给药途径在连续给药3 d后的药效学差异。分别ig给予YQFM 0.867、1.734、3.468 g/kg,尾iv给予YQFM 0.867、1.734 g/kg,评价两种给药途径在不同给药浓度时的药效差异。结果与模型组比较,急性及慢性心肌梗死小鼠iv给药3 d或ig给药7 d后,血清LDH和CK水平显著降低(P<0.05、0.01、0.001),说明iv给药组起效时间早于ig给药组。连续给药3 d后,iv给药组在改善心脏功能、降低LDH、CK水平,提高SOD活性方面均显著优于ig给药组;两种给药途径均可改善心肌梗死小鼠心脏病理组织形态。相同给药剂量下,iv给药组较ig给药组对急性或慢性心肌梗死小鼠的保护作用更加显著。结论iv给予YQFM对于急性或慢性心肌梗死模型小鼠的改善作用较ig给药途径更加迅速、高效。

Objective To compared the protective effects of Yiqi Fumai Lyophilized Injection(YQFM)against myocardial infarction mice through intravenous injection and intragastric administration.Methods Acute myocardial infarction model was induced by ligation of the left anterior descending coronary artery,and the model of chronic myocardial infarction was obtained after one week raise.The maximum clinical equivalent dose of YQFM was given by intravenous and intragastric administration respectively.Serum LDH and CK levels were measured at different dosing times,and the onset time of the two routes of administration was compared.The cardiac ultrasound,superoxide dismutase(SOD)and cardiac HE staining were performed to evaluate the pharmacodynamic differences between two routes of administration after 3 days of continuous administration.Evaluate the differences in efficacy between two administration routes at different administration concentrations.Results The acute and chronic myocardial infarction model mice were iv administered for 3 days or 7 days after ig administration.After that,serum LDH and CK levels were significantly decreased(P<0.05,0.01 and 0.001),indicating that the onset of iv administration was earlier than the ig administration group.After 3 days of continuous administration,intravenous injection of YQFM was significantly superior to the intragastric administration group in improving cardiac function,decreasing LDH,CK levels,and increasing SOD activity.Both can improve the pathological morphology of model mice heart.Under the same dose,iv administration group was more effective in treatment of acute or chronic myocardial infarction than the ig administration group.Conclusion Compared with ig administration,intravenous injection of YQFM has advantages of earlier onset time and more effective protection in acute or chronic myocardial infarction.