基于网络药理学探讨黄芪治疗慢传输型便秘作用机制

The Mechanism of Astragalus Membranaceus in Treatment of Slow Transit Constipation Based on Network Pharmacology

目的:基于网络药理学探讨黄芪治疗慢传输型便秘(STC)的作用机制。方法:应用TCMSP及UniPort数据库检索并筛选黄芪的活性成分及其对应的靶基因,通过GeneCards、OMIM数据库获取STC的靶点基因,并筛选黄芪有效活性成分与STC的交集靶点,使用Cytoscape 3.7.2软件绘制蛋白互作(PPI)网络图,预测其关键靶点;使用DAVID数据库对交集靶点进行GO分析及KEGG分析。结果:共得到黄芪有效活性成分16个,作用靶点172个,获得STC靶点1824个,黄芪与STC的共同靶点有113个。PPI分析得到Dgree值较大的17个靶点,说明可能是黄芪治疗STC的关键靶点。KEGG分析得到84条通路,包括癌症信号通路、PI3K/Akt信号通路、TNF信号通路、MAPK信号通路等。这说明黄芪中槲皮素等16个活性成分主要通过AKT1、TP53、IL6、VEGFA、CASP3、MAPK8等113个作用靶点作用于PI3K/Akt信号通路、MAPK信号通路等84条通路发挥治疗慢传输型便秘的作用。结论:黄芪可通过多成分、多靶点、多通路发挥促进细胞增殖、诱导凋亡、减轻炎症反应等药理作用干预STC,为进一步分子机制研究提供科学依据。

Objective:To explore the mechanism of Astragalus membranaceus in the treatment of slow transit constipation(STC)based on network pharmacology.Methods:The active components and corresponding target genes of Astragalus membranaceus were searched and screened by TCMSP database and Uniport database.Use GeneCards and OMIM database to obtain STC related targets,and screen intersection targets of active ingredients of Astragalus membranaceus and STC,the Cytoscape 3.7.2 software was used to draw the target protein-target protein interaction network,and predict its key targets.The intersection targets was analyzed by GO and KEGG using DAVID database.Results:The research screened out that 16 active ingredients,172 corresponding targets,and 1824 STC targets.There are 113 common targets of Astragalus membranaceus and STC.PPI analysis revealed 17 targets with larger dgree values,indicating that Astragalus membranaceus may be a key target for STC treatment.KEGG analysis revealed 84 pathways,including Cancer signaling pathway,PI3 K/Akt signaling pathway,TNF signaling pathway,MAPK signaling pathway,etc.The 16 components might play analgesic effects through 84 signal pathways,such as PI3 K/Akt signaling pathway and MAPK signaling pathway,which to be regulated by 113 related targets,such as AKT1、TP53、IL6、VEGFA、CASP3、MAPK8.Conclusion:Astragalus membranaceus can promote cell proliferation,induce apoptosis and reduce inflammatory reaction through multi-component,multi-target and multi-channel pharmacological effects,which can provide a certain basis for further molecular mechanism research.