摘要
目的:探索活血散结中药复方含药血浆对碱性成纤维细胞生长因子(bFGF)干预下兔视网膜色素上皮(RPE)细胞Bax、Bcl-2蛋白表达的影响。方法:选用对数生长期兔RPE细胞,分为正常血浆组、bFGF组、bFGF+BGJ398组、bFGF+含药血浆组,Western blotting法检测各组兔RPE细胞Bcl-2、Bax蛋白表达情况,观察活血散结中药复方含药血浆作用后RPE细胞Bcl-2、Bax蛋白在兔RPE细胞中的表达。结果:bFGF+含药血浆组与bFGF比较,Bax蛋白表达明显增强,Bcl-2蛋白表达明显抑制,差别均有显著统计学意义(P<0.01)。结论:活血散结中药复方含药血浆作用于bFGF干预下所致增生性玻璃体视网膜病变(PVR)的细胞模型,可促进RPE细胞Bax蛋白的表达,同时抑制Bcl-2蛋白的表达,使Bax/Bcl-2构成比升高。
Objective:To explore the effects of plasma containing invigorating blood and dissipating masses Chinese medicine on Bax and Bc1-2 protein expression of rabbit RPE cell under bFGF intervention.Methods:Select the rabbit RPE cells in log phase.The experiment objects were divided into the normal plasma group,the bFGF group,the bFGF+BGJ398 group,the bFGF+drug-containing plasma group.Western Blotting method was used to detect the Bcl-2,Bax protein expression condition in rabbit RPE cells and the Bcl-2,Bax protein expression condition in rabbit RPE cells after being acted by invigorating blood and dissipating masses Chinese medicine compound was observed.Results:Compared with bFGF group,Bax protein expression of bFGF+drug-containing plasma group was strengthened evidently,and Bcl-2 expression inhabitation was lightened,and the difference was statistically significant(P<0.01).Conclusion:Plasma containing invigorating blood and dissipating masses Chinese medicine acts on PVR cell model caused by bFGF intervention,promotes Bax protein expression and inhibits Bcl-2 protein expression,reducing the constituent ratio of Bax/Bcl-2.
作者
蔡娅仙
罗萍
彭俊
李建超
彭抿
吴权龙
彭清华
Cai Yaxian;Luo Ping;Peng Jun;Li Jianchao;Peng Pi;Wu Quanlong;Peng Qinghua(Hunan University of Chinese Medicine,Changsha 410208,China;Xingsha Hospital of Changsha County,Changsha 413200,China;The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;Xi'an Hospital of Traditional Chinese Medicine,Xi'an 710000,China)
出处
《亚太传统医药》
2021年第7期20-22,共3页
Asia-Pacific Traditional Medicine
基金
国家自然科学基金面上项目(81774372)
湖南省中医药管理局科研项目(201622)
湖南省研究生科研创新项目(CX2016B377、CX2017B432)
中医药防治五官科疾病湖南省重点实验室建设项目(2017TP1018)
长沙市科技计划(kel704005)
国家中医药管理局中医眼科学重点学科建设项目(ZK1801YK015)
关键词
活血散结中药复方
含药血浆
视网膜色素上皮细胞
Bcl-2
BAX
Invigorating Blood and Dissipating Masses Chinese Medicine Compound
Plasma Containing Drugs
RPE cell
Bcl-2
Bax