川芎茶调散8味组成药中15种无机元素分析

Analysis of 15 Inorganic Elements of 8 Components in Chuanxiong Chatiao Powder

目的:研究川芎茶调散8味组成药中15种无机元素的特性。方法:建立测定川芎茶调散8味组成药中15种无机元素含量的ICP-MS法。建立川芎茶调散8味组成药中15种无机元素的特征图谱;运用SIMCA-PCA分析8味组成药之间的差异,并结合主成分分析找出川芎茶调散中川芎、羌活、白芷、细辛、荆芥、防风、薄荷发挥祛风止痛功效的共有无机元素。运用Student-Newman-Keuls比较这7味药共有功效无机元素之间的差异。结果:建立的川芎茶调散8味组成药中15种无机元素图谱的特征性强。在SIMCA-PCA的分散点3D图中,甘草与其余7味药之间差异明显。主成分分析显示川芎、羌活、白芷、细辛、荆芥、防风、薄荷发挥祛风解表功效的共有无机元素为Mg、K、S;在7味药之间,Mg、S的差异无统计学意义,K的差异具有统计学意义。结论:建立的测定川芎茶调散8味组成药中15种无机元素含量的ICP-MS方法准确、可靠、重现性好。川芎茶调散8味组成药的无机元素特征图谱差异大,共有的功效无机元素为Mg、K、S。

Objective:To study the characteristics of fifteen inorganic elements in eight components of Chuanxiong Chatiao Powder(CCS).Methods:ICP-MS method was developed for the determination of fifteen inorganic elements in eight components of CCS.The fingerprints of fifteen inorganic elements were developed in eight component medicine of CCS.SIMCA-PCA was used to analyze the differences among eight component medicines,and inorganic elements of common efficacy of expelling wind to relieve pain were analyzed by principal component analysis(PCA)among Chuanxiong,Notopterygh,Angelicae Dahuricae,Asari,Schizonepetae,Saposhnikoviae,Menthae Haplocalycis and Glycyrrhizae.Student-Newman-Keuls was used to compare the differences of inorganic elements of common efficacy among these seven medicines.Results:The developed fingerprints of fifteen inorganic elements were strongly characteristics in eight component medicine of CCS.There were significant difference between Glycyrrhizae and others.The common inorganic elements were Mg,S and K based on the efficacy of expelling wind to relieve superficies among Chuanxiong,Notopterygh,Angelicae Dahuricae,Asari,Schizonepetae,Saposhnikoviae,Menthae Haplocalycis and Glycyrrhizae.Mg,S were no significant difference and K were significant difference among seven medicines.Conclusion:The ICP-MS method was accurate,reliable and reproducible for the determination of fifteen inorganic elements in eight components of CCS.The fingerprints of inorganic elements were different in eight component medicine of CCS,and the inorganic elements of common efficacy were Mg,K and S.