不同波长流动分析仪法检测中性石灰性土壤中的有效磷含量

Study on the Available Phosphorus Content of Difference Wavelength on Flow Analyzer Method to Detect Neutral and Calcareous Soil

为探究连续流动分析法,采用660 nm、880 nm波长检测中性、石灰性土壤有效磷含量差异。通过选择本地30个中性、石灰性土壤样品,采用农业行业标准(NY/T 1121.25-2012)连续流动分析法,利用660 nm、880nm波长检测有效磷含量,与常规紫外分光光度法对比。1)当有效磷含量较低时(小于10mg/kg),传统分光光度法比色检测的有效磷浓度高于660 nm与880 nm波长连续流动分析仪法的检测值;2)880 nm波长比660 nm波长增益值低,连续流动仪880 nm波长比660 nm波长的有效磷含量检测值低;3)当有效磷含量大于10 mg/kg时,应用流动分析仪660 nm、880 nm与常规检测880 nm三种结果无显著差异。研究发现有效磷质量分数大于10mg/kg时,应用流动分析仪660nm、880nm与常规检测880nm三种结果无显著差异。当有效磷质量分数小于10mg/kg时,常规分光光度检测建议使用无磷的活性炭粉震荡吸附后再比色。

In order to explore the continuous flow analysis method,the wavelengths of 660 nm and 880 nm were used to detect the difference in the content of available phosphorus in neutral and calcareous soils.By selecting 30 local neutral and calcareous soil samples,using the agricultural industry standard(NY/T 1121.25-2012)continuous flow analysis method,using 660 nm and 880 nm wavelengths to detect the effective phosphorus content,compared with conventional ultraviolet spectrophotometry.1)When the effective phosphorus content is low(less than 10 mg/kg),the effective phosphorus concentration detected by traditional spectrophotometry is higher than the detection value of 660 nm and 880 nm wavelength continuous flow analyzer method;2)880 nm wavelength The gain value is lower than the wavelength of 660 nm,and the effective phosphorus content detection value of the continuous flow meter at 880 nm wavelength is lower than that of the 660 nm wavelength;3)When the effective phosphorus content is greater than 10 mg/kg,use flow analyzer 660 nm,880 nm and conventional There was no significant difference between the three results at 880 nm.The study found that when the effective phosphorus mass fraction is greater than 10 mg/kg,there is no significant difference between the three results of using a flow analyzer at 660 nm,880 nm and conventional detection at 880 nm.When the effective phosphorus mass fraction is less than 10 mg/kg,conventional spectrophotometric detection is recommended to use phosphorus-free activated carbon powder after shaking and adsorption before color comparison.