对叶百部碱对HCT116及NCM460细胞活性的影响

Effect of Tuberostemonine on the activity of HCT116 cells and NCM460 cells

目的:探讨对叶百部碱对人结肠癌HCT116细胞及人正常结肠NCM460细胞活性的影响。方法:选择人结肠癌HCT116细胞,利用对叶百部碱(0、25、50、75、100、125 mg·L^(-1))进行干预24、48、72 h,选择人正常结肠NCM460细胞,利用对叶百部碱(0、10、25、50、75、100、125 mg·L^(-1))进行干预24 h,分别采用噻唑蓝(MTT)比色法检测对叶百部碱对HCT116及NCM460细胞的活性的影响;结果:与正常组比较,对叶百部碱组给药干预24、48、72 h人结肠癌HCT116细胞存活率显著降低,细胞增殖抑制率显著升高(P<0.01);与正常组比较,对叶百部碱组给药干预24 h人正常结肠NCM460细胞存活率无显著差异(P>0.05)。结论:在一定实验条件下,对叶百部碱(10、25、50、75、100、125 mg·L^(-1))对人正常结肠NCM460细胞无明显细胞毒性作用;对叶百部碱(25、50、75、100、125 mg·L^(-1))可抑制人结肠癌HCT116细胞增殖。

Objective:To investigate the effect of Tuberostemonine on the activity of human colon cancer HCT116 cells and human normal colon NCM460 cells.Methods:Human colon cancer HCT116 cells were selected and treated with foliperine(0,25,50,75,100,125 mg·L^(-1))for 24,48,72 h.Normal human colon NCM460 cells were selected.After 24 hours of intervention with Tuberostemonine(0,10,25,50,75,100,125 mg·L^(-1)),the effects of Tuberostemonine on the activity of HCT116 and NCM460 cells were detected by MTT colorimetric method.Results:Compared with the blank group,the survival rate of human colon cancer HCT116 cells was significantly decreased and the cell proliferation inhibition rate was significantly increased after 24,48 and 72 hours of treatment with Tuberostemonine group(P<0.01).Compared with the blank group,there was no significant difference in survival rate of normal colon NCM460 cells in the treatment group for 24 h(P>0.05).Conclusions:Under certain experimental conditions,Tuberostemonine(10,25,50,75,100,125 mg·L^(-1))has no obvious cytotoxic effect on NCM460 cells of normal colon.Tuberostemonine(25,50,75,100,125 mg·L^(-1))can inhibit the proliferation of human colon cancer HCT116 cells.