尿酸盐水解酶假基因在子痫前期胎盘组织的表达及其分子机制

Expression of urate hydrolase pseudogene in placentae tissues of preeclampsia and its molecular mechanism

目的探讨长链非编码RNA(Lnc RNA)尿酸盐水解酶假基因(URAHP)在子痫前期胎盘组织中的异常表达及其对滋养层细胞增生、侵袭的影响。方法选取子痫前期及正常孕妇胎盘组织6例,其中PE组纳入子痫前期患者3例,对照组纳入正常妊娠孕妇3例。筛选2组差异表达LncRNA和miRNA;分析LncRNA URAHP在2组胎盘组织中的差异表达。采用CCK-8法,分析URAHP对滋养层细胞增生及侵袭的影响。测定URAHP和吻素受体(KISS1R)在JAR、JET-3、HTR-8/SVneo细胞系中的表达。结果通过分析鉴定了205个差异表达基因,其中56个上调,149个下调。与正常妊娠相比,子痫前期患者胎盘组织中假基因URAHP表达增加,差异有统计学意义(P<0.05)。URAHP的下调可改变JAR/JET-3细胞增殖能力;URAHP过表达可促进HTR-8/SVneo细胞增殖。KISS1R是URAHP在子痫前期胎盘中的共表达基因。结论假基因URAHP可能与子痫前期发病有关。

Objective To investigate the abnormal expression of long non-coding RNA(LncRNA)urate hydrolase pseudogene(URAHP)in the placental tissue of preeclampsia and its effects on proliferation and invasion of trophoblast cells.Methods Six cases of preeclampsia and normal maternal placental tissue were selected,among whom three patients with preeclampsia were selected as PE group,and three normal pregnant women as control group.The differentially expressed LncRNAs and miRNAs in the two groups were screened;the differential expression of LncRNA URAHP in the placental tissues of the two groups was analyzed.CCK-8 method were used to analyze the effects of URAHP on trophoblastic proliferation and invasion.The expressions of URAHP as well as KISS1R in JAR,JET-3 and HTR-8/SVneo cell lines were determined.Results A total of 205 differentially expressed genes were identified,among which 56 were up-regulated and 149 were down-regulated.The expression of URAHP pseudogenes was significantly increased in placental tissues of preeclampsia patients compared with those of the normal pregnant women(P<0.05).Downregulation of URAHP could change the proliferation ability of JAR/Jet-3 cells;the overexpression of URAHP promoted the proliferation of HTR-8/SVneo cells.KISS1R was the co-expression gene of URAHP in the placenta of preeclampsia.Conclusion Pseudogene URAHP may be associated with the onset of preeclampsia.