microRNA-195在弥漫大B细胞淋巴瘤患者中的表达及临床意义

Expression and Clinical Significance of MicroRNA-195 in Patients with Diffuse Large B Cell Lymphoma

目的:探讨微小RNA 195(miR-195)在弥漫大B细胞淋巴瘤(DLBCL)患者中的表达及其临床意义。方法:选取本院近4年诊治的60例DLBCL患者,并选取同时期同年龄段于本院行健康体检的健康人群30例作为对照,采用实时荧光定量PCR(qRT-PCT)分别测定两组患者miR-195的表达量,分析其表达与患者临床病理特征及患者生存时间的关系。结果:miR-195在DLBCL患者中的表达量较对照组显著降低(P<0.001);miR-195的表达水平与DLBCL患者肿瘤直径、IPI指数评分及Ann Arbor分期密切相关;高表达miR-195的DLBCL患者总体生存时间显著高于低表达患者(P<0.001)。结论:miR-195表达在DLBCL患者中的表达降低,miR-195与DLBCL患者肿瘤特征密切相关,高表达miR-195的DLBCL患者可获得更长的总体生存时间。

Objective:To investigate the expression and clinical significance of microRNA-195 in patients with diffuse large B cell lymphoma(DLBCL).Methods:Sixty patients with DLBCL were selected from nearly four years in our hospital,and at the same time 30 healthy people with physical examination of the same period and with the same age in our hospital were choosed as control group.Real-time fluorescent quantitative PCR(RT-qPCR)was used to detect the miR-195 expression of the patients and controls,the relationship between miR-195 expression and clinicopathological characteristics of DLBCL and survival time of patients was analyzed.Results:The expression level of miR-195 in DLBCL patients was significantly lower than that in the controls(P<0.001).The expression level of miR-195 closely related with tumor diameter,IPI score and Ann Arbor stage of patients with DLBCL.Overall survival(OS)time of DLBCL patients with highly expressed miR-195 was significantly longer than that of patients with low expression(P<0.001).Conclusion:miR-195 expression decrease in DLBCL patients,and miR-195 closely relates with tumor characteristics of patients with DLBCL.DLBCL patients with higher expression of miR-195 show longer overall survival time.