一种天然复合活性物的抗炎功效及其机制研究

Study on Anti-Inflammatory Effect and Mechanism of a Natural Compound Active

以依克多因、羟基积雪草甙、姜黄挥发油、姜黄素组成的天然复合活性物(Natural Compound Active,NC)为研究对象,通过体外细胞及体内动物试验对其抗炎功效及其机制进行研究。结果显示,在LPS诱导的巨噬细胞炎症模型上,NC有效降低NO含量。进一步通过TPA诱导耳肿胀模型测试,NC有效降低由TPA诱导的耳肿胀,抑制率达55%以上,其可能是通过抑制炎症反应过程中的关键性细胞因子IL-6、TNF-α、MIP-2、MMP-1的分泌和释放从而发挥抗炎作用,此外,NC能显著提高体内的超氧化物歧化酶SOD含量,及时清除氧自由基及脂质过氧化物MDA,有助于降低皮肤炎症反应,减少炎症对皮肤组织的损害。天然复合活性物(NC)作为一种天然来源的具有抗炎功效的活性原料,可应用于皮肤护理领域。

The anti-inflammatory effect and mechanism of a natural compound active(NC)composed by ectoin,madecassoside,turmeric volatile oil and curcumin were studied by in vitro cell and in vivo animal experiments.The results showed that in LPS induced macrophage inflammation model,the NC could effectively reduce the NO content.Furthermore,through the TPA induced ear swelling model test,the NC can effectively reduce the TPA induced ear swelling,the inhibition rate was more than 55%,which may be resulted from the inhibition of key cytokines IL-6,TNF-α,MIP-2 and MMP-1.In addition,the NC active can significantly increase the content of superoxide dismutase(SOD)in vivo,clear oxygen free radicals and malondiadehyde(MDA)in time,which is helpful to reduce the inflammatory reaction of skin and reduce the damage of inflammation to skin tissue.As a kind of natural anti-inflammatory active material,this natural compound(NC)can be used in the field of skin care.