基于偏最小二乘法分析猪牙皂不同组分对大鼠脑缺血再灌注损伤的影响

Effects of Gleditsia Sinensis Lam. on Cerebral Ischemia Reperfusion Injury in Rats Based on Partial Least Squares Method

目的探讨猪牙皂对脑缺血再灌注损伤大鼠的保护作用,及其药效部位。方法实验分6组,即假手术组、模型组、尼莫地平组、皂苷组、皂苷+萃取物组、萃取物组。建立大鼠脑缺血再灌注模型,进行大鼠神经功能评分,测量大鼠脑组织含水量,采用ELISA法检测大鼠脑缺血侧脑组织及血清中白介素-1β(interleukin-1β,IL-1β)、白介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、丙二醛(malondialdehyde,MDA)、一氧化氮(nitric oxide,NO)的含量和一氧化氮合酶(nitric oxide synthase,NOS)、总超氧化物歧化酶(superoxide dismutase,SOD)的活性;最后借助偏最小二乘法和方差分析对比猪牙皂不同组分的作用。结果与模型组比较,各给药组神经功能评分均下降,大鼠脑组织含水量、脑组织及血清中的IL-1β、IL-6、TNF-α、MDA、NO含量均降低,NOS活性被抑制,SOD活性显著增强。偏最小二乘法分析可得:皂苷4.5 mg·kg^(-1)对大鼠缺血性再灌注模型的保护作用强于皂苷+萃取物6.1 mg·kg^(-1)与萃取物1.6 mg·kg^(-1)。结论猪牙皂皂苷对脑缺血再灌注损伤大鼠具有一定的治疗作用,其作用机制与抗炎、抗氧化应激作用有关。

OBJECTIVE To explore the protective effect of Gleditsia sinensis Lam.on brain injury in rats following cerebral ischemia reperfusion injury,and the effect of the drug-effect site.METHODS There were 6 groups:sham operation group,model group,nimodipine group,saponin group,saponin+volatile oil group,volatile oil group.The rat model of cerebral ischemia-reperfusion was established.The neurological function score of rats was measured.The water content of brain tissue was measured.The contents of interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),malondialdehyde(MDA),nitric oxide(NO)and the activities of nitric oxide synthase(NOS)and total superoxide dismutase(SOD)in the cerebral tissue and serum of cerebral ischemia rats were determined by ELISA.Finally,partial least squares method was used to comprehensively analyze the cerebral protective effect of different components of Gleditsia sinensis Lam.on model rats.RESULTS Compared with the model group,the neurological function score of each group decreased,and the brain water content,IL-1β,IL-6,TNF-α,MDA and NO content in the brain tissue and serum of the rats were all decreased.The activity of NOS was inhibited and SOD activity was significantly enhanced.Partial least squares analysis showed that the protective effect of saponin 4.5 mg·kg^(-1)on the ischemic reperfusion model of rats was stronger than that of saponin+volatile oil 6.1 mg·kg^(-1)and volatile oil 1.6 mg·kg^(-1).CONCLUSION Saponin of Gleditsia sinensis Lam.has a certain therapeutic effect on cerebral ischemia-reperfusion injury in rats,its mechanism of action related to anti-inflammation,resisting oxidative stress.