桦木脑通过SREBP1抑制靶基因PIK3R3抗肝癌

Betulin on Anti-Hepatocellular Carcinoma through SREBP1 Inhibits the Target Gene PIK3R3

该文主要研究桦木脑通过固醇调节元件结合蛋白1(sterol-regulatory element binding proteins,SREBP1)抑制靶基因PIK3R3(phosphoinositide-3-kinase regulatory subunit 3)抗肝癌的分子机制。采用MTT法检测细胞增殖;流式细胞仪检测细胞周期和凋亡;qRT-PCR检测细胞周期蛋白、周期蛋白激酶和凋亡相关基因mRNA水平;采用RNA-seq并整合ChIP-Seq数据筛选SREBP1调控的凋亡相关基因;双荧光素酶报告系统检测SREBP1对凋亡基因的调控作用。结果显示,桦木脑能够显著抑制肝癌细胞增殖,诱导其凋亡,并且使肝癌细胞阻滞在G2/M期;桦木脑通过抑制SREBP1可直接下调凋亡基因PIK3R3的表达,促进细胞凋亡,进而抑制肝癌细胞增殖。

This study focused on the molecular mechanism of betulin on Anti-hepatocellular carcinoma through SREBP1 inhibition of target gene PIK3R3.Cell proliferation was detected by MTT assay.Cell cycle and apoptosis were detected by flow cytometry.mRNA expression levels of cyclins,cyclin dependent kinase and apoptosis-related genes were detected by qRT-PCR.SREBP1-regulated apoptosis-related genes were screened by integration of RNA-seq and ChIP-Seq data.The regulation of SREBP1 on the apoptotic gene was detected by DualLuciferase reporter system.The results showed that betulin can significantly inhibit the proliferation of hepatocellular carcinoma cells,induce apoptosis,and block hepatocellular carcinoma cells in G2/M phase.Betulin can directly down-regulate the expression of apoptosis gene PIK3R3 to promote apoptosis and inhibit the proliferation of Hepatocellular Carcinoma cells.