姐妹染色体过早分离基因5黏附相关因子B(PDS5B)下调Wnt5a表达抑制A549人肺癌细胞增殖

PDS5B inhibits the proliferation of A549 human lung cancer cells via downregulation of Wnt5a

目的探讨姐妹染色体过早分离基因5黏附相关因子B(PDS5B)对A549肺癌细胞株生物学功能的影响及可能的分子机制。方法沉默或过表达A549人肺癌细胞的PDS5B后,噻唑蓝(MTT)法及集落形成实验检测肺癌细胞的增殖情况;划痕实验及TranswellTM检测细胞迁移的情况;Western blot法检测A549细胞PDS5B及无翅基因5a(Wnt5a)的蛋白表达;共同转染PDS5B小干涉RNA(siRNA)及Wnt5a siRNA,TranswellTM检测细胞迁移的情况。结果与转染阴性对照组相比,转染PDS5B siRNA后,PDS5B蛋白表达明显下降且A549细胞增殖能力、细胞集落形成率、迁移能力均明显提高,Wnt5a的表达升高;过表达PDS5B后,则结果相反;共转实验显示Wnt5a可以抵抗PDS5B对A549细胞的抑制作用。结论PDS5B通过下调Wnt5a表达抑制肺癌细胞增殖。

Objective To investigate the effect of PDS5B on the biological function of A549 human lung cancer cells and possible molecular mechanism.Methods The proliferation of lung cancer cells was detected by MTT assay and colony formation assay after silencing or overexpressing PDS5B of A549 cells.The cell migration was detected by scratch assay and TranswellTMassay.The protein expression of PDS5B and Wnt5a in A549 cells was detected by Western blot analysis.Cell migration was detected by TranswellTMafter PDS5B small interference RNA(siRNA)and Wnt5a siRNA were co-transfected.Results Compared with the negative control group,the protein expression of PDS5B decreased significantly after transfected with PDS5B siRNA.The proliferation ability,colony formation rate and migration ability of A549 cells significantly improved,and the expression of Wnt5a was increased.The opposite results were observed after PDS5B over-expression.The co-transfer experiment showed that Wnt5a could resist the inhibition of A549 cells by PDS5B.Conclusion PDS5B inhibits lung cancer cell proliferation by down-regulating Wnt5a expression.