1,341名献血员HBsAg检测及筛检方法的探讨

Comparison of Sensitivity of Screening Methods for HBsA9 in 1341 Blood Donors

近年来国内大部分医院对献血员都建立了病毒性肝炎的筛检制度。因此,有必要选择一种敏感、特异、简便、快速的检测方法。我们于1983年5月~6月,对本市1,341名献血员进行了谷丙转氨酶(SGPT)及乙型肝炎表面抗原(HBsAg)的检测。

1,341 blood donors(263 donors from urban and 1,078 donors from rural area were screened for HBsAg by RPHA and ELISA methods.The over all incidence of HBsAg positive was 7.9%(106/1,341).For male it was 8.34%and for female 7.19%.In urban area it was 11.03%and in rural area 7.14%.There was no correlation between the incidences of HBsAg positive rates in sex,age donate frequency and blood groups.1,042 serum specimens were tested for SGPT by Reitmen’s method.The frequency of abnormal SGPT was 4.41%.Out of the 46 donors with abnormal SGPT,only 6 was HBsAg-positive(13.04%)and the other 40 were HBsAg-negative which were most likely having other type of liver diseases.In comparison of RPHA with ELISA methods for the detection of HBsAg in 1,341 serum specimens,the frequency(7.16%)of HBsAg positive by ELISA method w s 1.88 times higher than that(3.8%)by RPHA method.The ELISA method appeared to be more sensitive than RPHA method.It is emphasized that the screening of blood donors for HBsAg is very important.Some suggestions for improving the work of blood bank in hospital were advocated.