YBX1和KPNA2在肺鳞癌中的表达及其临床意义

Expression and clinical significance of YBX1 and KPNA2 in lung squamous cell carcinoma

目的:探讨Y-盒结合蛋白1(YBX1)和核转运蛋白α2(KPNA2)在肺鳞癌中的表达及其临床意义。方法:选取本院2015年1月至2017年5月收治的137例肺鳞癌患者进行研究,术中收集肺鳞癌组织及相应癌旁组织,采用免疫组化染色法检测肺鳞癌组织、癌旁组织中YBX1、KPNA2表达情况,采用乘积极限(K-M)法进行生存分析,Log-rank检验比较显著性;COX回归模型分析影响肺鳞癌患者预后生存状态的因素。结果:与癌旁组织相比,肺鳞癌组织中YBX1、KPNA2阳性表达率较高(P<0.05);肺鳞癌组织中YBX1、KPNA2表达与肿瘤分化程度、临床分期、淋巴结转移有关(P<0.05);Spearman相关分析显示,肺鳞癌组织中YBX1与KPNA2表达呈正相关(r=0.508,P<0.05);随访期内肺鳞癌患者死亡率为37.23%,与YBX1、KPNA2阴性表达者相比,YBX1、KPNA2阳性表达者死亡率较高(P<0.05),总生存期较短(P<0.05);多因素COX分析结果显示,YBX1阳性表达、KPNA2阳性表达、肿瘤分期为Ⅲ期、伴淋巴结转移是影响肺鳞癌患者预后生存状况的独立危险因素(P<0.05)。结论:肺鳞癌组织中YBX1、KPNA2阳性表达与患者肿瘤分化程度、临床分期、淋巴结转移有关,YBX1、KPNA2有望用于评估肺鳞癌患者病情演变及预后状况。

Objective:To investigate the expression and clinical significance of Y-box binding protein 1(YBX1)and karyopherin alpha 2(KPNA2)in lung squamous cell carcinoma.Methods:A total of 137 patients with lung squamous cell carcinoma admitted to our hospital from January 2015 to May 2017 were selected for the study,lung squamous cell carcinoma tissues and corresponding adjacent tissues were collected during the operation.Immunohistochemical staining was used to detect the expression of YBX1 and KPNA2 in lung squamous cell carcinoma and adjacent tissues,and the expression of YBX1 and KPNA2 in lung squamous cell carcinoma and adjacent tissues was compared;the relationship between the expression of YBX1 and KPNA2 in lung squamous cell carcinoma and the clinicopathological features was analyzed;the product limit(K-M)method was used for survival analysis,Log-rank test was used to compare the significance;COX regression model was used to analyze the factors influencing the prognostic of patients with lung squamous cell carcinoma.Results:In contrast to parcancer tissue,YBX1,KPNA2 positive expression in pulmonary squamous carcinoma(P<0.05);YBX1,KPNA2 expression in pulmonary squamous carcinoma tissue was related with degree of tumor differentiation,clinical staging,lymph node metastasis(P<0.05).The Spearman-related analysis shows that,YBX1 was positively associated with KPNA2 expression in pulmonary squamous carcinoma tissue(r=0.508,P<0.05).During the follow-up period,the mortality of lung squamous cell carcinoma was 37.23%.In contrast to the YBX1,KPNA2-negative expression person,High YBX1,KPNA2-positive expression mortality(P<0.05),Short total survival period(P<0.05);Multi-factor COX analysis results showed that,YBX1 positive expression,KPNA2 positive expression,the tumor stage was stageⅢ,and associated lymph node metastasis were independent risk factors affecting the prognosis survival of patients with pulmonary squamous carcinoma(P<0.05).Conclusion:The positive expressions of YBX1,KPNA2 in lung squamous carcinoma tissue is related to degree of tumor differentiation,clinical stage and lymph node metastasis.YBX1,KPNA2 expected for evaluation the evolution and prognosis of pulmonary squamous carcinoma patients.