桂枝茯苓丸缓解子宫内膜异位症大鼠疼痛的作用与机制研究

Effect and mechanism of Guizhi Fuling pills on alleviating pain in rats with endometriosis

目的探讨桂枝茯苓丸对子宫内膜异位症大鼠疼痛的治疗作用与机制。方法测定桂枝茯苓丸对子宫内膜异位症大鼠疼痛阈值、DRG神经元损伤、子宫内膜异位组织神经纤维密度及细胞中NGF/Erk信号通路的影响。结果桂枝茯苓丸能显著提高子宫内膜异位症大鼠的热辐射痛和机械痛阈值(P<0.05),有效改善DRG神经元损伤,降低异位内膜组织中神经纤维NF200的密度,并能明显抑制NGF受体的表达和Erk的磷酸化水平。结论桂枝茯苓丸有效缓解子宫内膜异位症疼痛,可能是通过改善DRG神经元损伤,降低神经纤维密度,调控NGF/Erk信号通路发挥作用的。

Objective To investigate the therapeutic effect and mechanism of Guizhi Fuling pills on pain in rats with endometriosis.Methods The thresholds of pain,the damage of DRG neuron and density distribution of nerve fibers of rats with endometriosis were measured.The expressions of the key factors in NGF/Erk signaling pathway were also detected.Results Guizhi Fuling pills can significantly increase threshold values of the thermal radiation pain and mechanical pain of rats with endometriosis(P<0.05),effectively repair the damage of DRG neuron and reduce the density of nerve fiber NF200 in endometrial tissues.It also significantly inhibited the expression level of NGF receptor and the phosphorylation of Erk in the NGF/Erk signaling pathway.Conclusion Guizhi Fuling pills can effectively alleviate the pain of endometriosis,which may be through improving the damage of DRG neurons,reducing the density of nerve fibers and regulating the NGF/Erk signaling pathway.