悬灸对湿热痹阻型RA大鼠类风湿因子表达影响的研究

Study on Rheumatoid Factor of Suspended Moxibustion for Dampness-heat Type RA Model Rats

目的观察悬灸对RA模型大鼠双侧踝关节温度及类风湿因子(RF)表达的影响。方法60只Wistar大鼠随机分出空白组(12只),正常饲养,其余复制湿热痹阻型RA大鼠模型,造模成功后随机分为模型组、温和灸组、回旋灸组和雀啄灸组,每组12只。模型组不处理,不同悬灸组给予不同悬灸法,治疗前后采用红外热成像仪检测双侧踝关节局部温度,治疗后采用ELISA法检测各组大鼠血清中RF的表达。结果与空白组相比,模型组大鼠双侧踝关节局部温度明显升高(P<0.01),悬灸治疗后温度明显降低(P<0.01);与温和灸组相比,回旋灸组和雀啄灸组大鼠左侧踝关节温度降低(P<0.01),右侧踝关节无明显差异(P>0.05),回旋灸与雀啄灸组比较无明显差异(P>0.05)。与空白组相比,模型组大鼠RF表达明显升高(P<0.01),悬灸治疗后大鼠RF表达明显降低(P<0.05),悬灸组间比较差异无统计学意义(P>0.05)。结论悬灸法能降低湿热痹阻型RA大鼠踝关节区温度,显著改善RA大鼠关节局部红肿症状,明显降低血清抗体RF表达,有效调整免疫功能紊乱。

Objective To observe the effect of suspended moxibustion for rheumatoid factor(RF)of dampness-heat type RA model rats.Methods Firstly,60 Wistar rats were randomly divided into control group,normal feeding.Secondly,the replication of dampness-heat type RA model,the rats were randomly divided into model group,mild moxibustion group,circling moxibustion group and bird pecking moxibustion group,12 rats in each group.The model group without treatment,different moxibustion group was given different suspended moxibustion method.The local temperature of bilateral ankle joints was measured by infrared thermography before and after treatment.RF content in serum of rats in each group was detected by ELISA method after treatment.Results compared with the blank group,the local ankle temperature in the model group was significantly increased(P<0.01),the temperature decreased significantly after treatment with suspended moxibustion(P<0.01),compared with mild moxibustion group,the temperature of the left ankle joint decreased in the circling moxibustion group and bird pecking moxibustion group(P<0.01),but there was no significant difference in the right ankle joint(P>0.05),and there was no significant difference between the circling moxibustion group and bird pecking moxibustion group(P>0.05).Compared with the blank group,the content of RF in the model group was significantly increased(P<0.01),which the RF content decreased significantly after treatment with suspended moxibustion(P<0.05),there was no significant difference between the suspended moxibustion groups(P>0.05).Conclusion Suspended moxibustion method can reduce the zone temperature of ankle,so as to improve the RA rat articular swelling symptoms,the serum antibody RF content of dampness-heat type RA model rats,adjusting the disorder of immune function.