MiR-126在脓毒症所致心肌损伤的作用研究

The roles of miR-126 in myocardial injury induced by sepsis

目的探讨miR-126在脓毒症心肌损伤中的作用并探讨其机制。方法在整体动物水平,将C57BL/6小鼠随机分为两组,即假手术组与脓毒症组。采用盲肠结扎穿刺术构建脓毒症小鼠模型,收集小鼠心脏组织,采用qRT-PCR检测小鼠心肌组织中miR-126的表达水平。在离体细胞水平,采用LPS刺激H9C2细胞,转染miR-126的模拟物与抑制物,采用CCK8检测H9C2细胞活力,采用LDH试剂盒检测H9C2细胞释放LDH的能力,观察miR-126对LPS所致H9C2细胞损伤的影响。结果与假手术组相比,脓毒症小鼠血清中LDH、CK-MB释放增加,提示脓毒症导致小鼠心肌损伤。小鼠心肌组织与LPS处理H9C2细胞中miR-126表达水平明显增加。当转染miR-126模拟物后H9C2细胞释放LDH减少,细胞活力增加;当转染miR-126抑制物后H9C2细胞释放LDH增加,细胞活力降低。结论miR-126可增强心肌细胞活力,改善脓毒症所致心肌损伤。

Objective To observe the expression of miR-126 in myocardial injury induced by sepsis and explore its mechanism.Methods In vivo,all C57 BL/6 mice were divided into two group randomly,namely sham group and sepsis group.Cecal ligation puncture(CLP)was used to establish sepsis animal model.qRT-PCR was utilized to detect the expression of miR-126 in heart.In vitro,qRT-PCR was used to detect the expression of miR-126 in H9C2 cells with the treatment of LPS.CCK8 and LDH assay were used to detect the cell viability and LDH release in H9C2 cells tranfected with miR-126 mimic or inhibitor before the treatment of LPS.Results The level of CK-MB and LDH were significantly increased in the sera of septic mice compared with that of the sham group.Besides,miR-126 expression was significantly elevated in the heart tissue of septic mice and H9C2 cells treated with LPS.MiR-126 is capable of enhancing cell viability and inhibiting the release of LDH in H9C2 cells.Conclusions MiR-126 could enhance cell viability and alleviate the myocardial injury induced by sepsis.