嗜盐性细菌培养检测海产品中副溶血性弧菌的效能评价与分析

Evaluation and analysis of the efficacy of halophilic bacterial culture in detecting Vibrio parahaemolyticus in seafood

目的评价和分析在大连地区海产品副溶血性弧菌检测中应用嗜盐性细菌培养检测的价值。方法采集46份海产品样本,研究时间为2023年7月至2023年10月,均来自大连地区,培养并分离嗜盐性细菌,实施菌株耐盐性试验并鉴定和分析副溶血性弧菌毒力,分析海产品副溶血性弧菌特性及检出情况。结果共计筛选出嗜盐菌株及耐盐菌株45株,20%盐度下获得率为44.44%,15%盐度下获得率为55.56%,50℃培养环境下获得率为11.11%。分离菌株在25%NaCl浓度下持续生长且10%NaCl浓度下菌株长势最好,检出17株中度嗜盐菌,耐盐菌株数量为28株。结论在大连地区海产品副溶血性弧菌检测中应用嗜盐性细菌培养检测可提高副溶血性弧菌检出率,而且毒力鉴定结果表明副溶血性弧菌毒力较强,具有引发食物中毒的潜在危险,相关部门必须加强海产品副溶血性弧菌检验以保证饮食安全。

Objective To evaluate and analyze the value of halophilic bacteria culture in the detection of Vibrio parahaemolyticus in seafood in Dalian area.Methods 46 samples of Marine products were collected from Dalian from July 2023 to October 2023,and halophilic bacteria were cultured and isolated.The salt-tolerance test was carried out to identify and analyze the virulence of Vibrio parahaemolyticus,and the characteristics and detection of Vibrio parahaemolyticus in Marine products were analyzed.Results A total of 45 halophilic and salt tolerant strains were screened,with a yield of 44.44%at 20%salinity,55.56%at 15%salinity,and 11.11%at 50℃cultivation environment.The isolated strains continued to grow at a concentration of 25%NaCl,and the best growth was observed at a concentration of 10%NaCl.Seventeen moderately halophilic bacteria were detected,with a total of 28 salt tolerant strains.Conclusion The detection rate of Vibrio parahaemolyticus in Marine products in Dalian area can be improved by using halophilic bacteria culture detection.The results of virulence identification show that Vibrio parahaemolyticus has strong virulence and potential risk of food poisoning.Relevant departments must strengthen the detection of Vibrio parahaemolyticus in Marine products to ensure dietary safety.