乙型肝炎病毒基因型分布及耐药突变位点分析

Hepatitis B virus genotype distribution and drug resistance mutation site analysis

目的探讨开封地区乙型肝炎病毒(HBV)基因型分布,基因耐药突变位点分布情况。方法选取开封地区2018年1月至2020年12月期间收集200例乙型肝炎表面抗原(HBsAg)阳性患者,采用基因测序技术检测患者基因型,对检测结果进行统计分析。并对患者血清中HBV-DNA进行核苷类似物(NAs)耐药基因位点分析。结果开封地区200例乙型肝炎表面抗原(HBsAg)阳性患者基因型C型179例,占比97.8%,B型4例,占比2.2%,未检测出B+C型及其他型;200例患者测序结果中26例检测出NAs基因耐药突变位点,基因C型患者耐药突变位点检出率高于基因B型患者。结论开封地区乙型肝炎表面抗原阳性患者HBV基因型以C型为主,B型较少,未检测到其它基因型;基因C型患者NAs耐药位点检出率高于B型。NAs耐药突变位点中共检出9种不同耐药突变位点组合模式,以单位点突变为主。

Objective To investigate the distribution of hepatitis B virus(HBV)genotypes and drug resistance mutation sites in Kaifeng area.Methods A total of 200 hepatitis B surface antigen(HBsAg)positive patients were selected from Kaifeng area from January 2018 to December 2020.The genotype of the patients was detected by gene sequencing technology,and the test results were statistically analyzed.Nucleoside analogues(NAs)resistance gene loci in serum HBVDNA of 200 untreated patients were analyzed.Results Among 200 HBsAg positive patients in Kaifeng area,179 cases were genotype C,accounting for 97.8%,4 cases were genotype B,accounting for 2.2%,and no B+C or other types were detected.Among the 200 patients,26 were detected NAs gene drug resistance mutation sites,and the detection rate of drug resistance mutation sites in patients with gene C was higher than that in patients with gene B.Conclusion Hepatitis B surface antigen positive patients in Kaifeng area of HBV genotype mainly C type,B type is less,no other genotype was detected.The detection rate of NAs resistance sites in genotype C was higher than that in genotype B.A total of 9 different combinations of NAs drug-resistant mutation sites were detected,mainly unit point mutation.