高效液相色谱-荧光检测法测定饮用水中的亚硝酸盐

Determination of nitrite in drinking water by high-performance liquid chromatography fluorescence detection method

目的评价高效液相色谱-荧光检测法测定饮用水中的亚硝酸盐应用价值。方法采集矿泉水、自来水与水源水作为水标本,应用高效液相色谱-荧光检测法测定,分析该方法检测优势及水标本中亚硝酸盐衍生物2,3-萘三唑稳定性。结果纯水中检测出亚硝酸盐浓度为0.6μg/L,峰形好且无杂质干扰;高效液相色谱-荧光检测法的检出限为0.3μg/L,定量限为1μg/L;亚硝酸盐衍生后,衍生物稳定性较高,在7 d内开展测定误差小;亚硝酸盐加标回收率在90.29%-102.60%之间,精密度在0.71%-3.66%。结论高效液相色谱-荧光检测法测定饮用水中的亚硝酸盐具有准确性、便捷性等特征,为饮用水安全监测提供重要的技术支持。

Objective To evaluate the application value of high-performance liquid chromatography fluorescence detection method for determining nitrite in drinking water.Method Collect mineral water,tap water,and source water as water samples,and use high-performance liquid chromatography fluorescence detection method to determine.Analyze the detection advantages of this method and the stability of nitrite derivative 2,3 naphthalene triazole in the water standard.Result The nitrite concentration detected in pure water was 0.6μg/L,with good peak shape and no impurity interference;The detection limit of high-performance liquid chromatography fluorescence detection method is 0.3μg/L,and the quantitative limit is 1μg/L;After derivatization with nitrite,the stability of the derivative is high,and the measurement error is small within 7 days;The recovery rate of nitrite spiking is between 90.29%-102.60%,and the precision is between 0.71%-3.66%.Conclusion The determination of nitrite in drinking water by high-performance liquid chromatography fluorescence detection method has the characteristics of accuracy and convenience,providing important technical support for drinking water safety monitoring.