高通量抗体芯片技术检测尖锐湿疣患者血清辅助性T细胞因子表达水平

The expression levels of serum helper T cell cytokines of condyloma acuminate based on high throughput cytokine antibody microarray

目的检测尖锐湿疣(condyloma acuminate,CA)患者血清中辅助性T淋巴细胞(helper T cell,Th细胞)相关细胞因子的表达水平,探讨其在CA发病机制中可能的作用,揭示CA患者全身的免疫状态。方法分别采集40例CA患者和20名健康对照者外周血,采用高通量抗体芯片技术检测CA患者及健康对照者外周血血清中Th细胞相关细胞因子表达水平。结果CA患者外周血血清中Th1型细胞因子干扰素(IFN)-γ、白细胞介素(IL)-2、肿瘤坏死因子(TNF)-α表达水平分别为(5.11±3.44)pg/ml、(4.44±2.53)pg/ml、(30.16±7.38)pg/ml,较健康对照组(9.5±3.77)pg/ml、(8.26±3.26)pg/ml、(37.06±12.16)pg/ml降低;Th2型细胞因子IL-4表达水平为(4.18±2.29)pg/ml,较健康对照组(2.07±1.10)pg/ml升高;Treg型细胞因子IL-10、转化生长因子(TGF)-β表达水平分别为(2.03±1.01)pg/ml、(904.67±317.91)pg/ml,较健康对照组(1.26±0.71)pg/ml、(648.96±180.34)pg/ml升高;Th17型细胞因子IL-17及IL-23表达水平分别为(1.39±0.23)pg/ml、(172.98±42.56)pg/ml,较健康对照组(1.62±0.44)pg/ml、(204.23±48.88)pg/ml降低;以上差异均有统计学意义(均P<0.05)。结论Th1/Th2、Th17/Treg型细胞因子交互作用失衡很可能是CA重要的免疫学发病机制之一。CA患者存在全身性的细胞免疫功能紊乱。

Objective To detect the expression levels of serum helper T cell(Th)related cytokines in patients with condyloma acuminate(CA).To explore the possible role of Th cell related cytokines in the pathogenesis of CA.To reveal the systemic immune status of CA patients.Methods Peripheral blood samples of 40 CA patients and 20 healthy controls were collected.The high throughput cytokine antibody microarray was used to detect the expression levels of Th cell related cytokines in the serum of CA patients and healthy controls.Results The expression levels of Th1 cytokines IFN-γ(5.11±3.44)pg/ml,IL-2(4.44±2.53)pg/ml and TNF-α(30.16±7.38)pg/ml in peripheral blood of CA patients were lower than the healthy control group(9.5±3.77)pg/ml,(8.26±3.26)pg/ml,(37.06±12.16)pg/ml,while the expression level of Th2 cytokine IL-4(4.18±2.29)pg/ml was higher than the healthy control group(2.07±1.10)pg/ml.The expression levels of Treg cytokines IL-10(2.03±1.01)pg/ml and TGF-β(904.67±317.91)pg/ml were higher than the healthy control group(1.26±0.71)pg/ml,(648.96±180.34)pg/ml,while the expression levels of Th17 cytokines IL-17(1.39±0.23)pg/ml and IL-23(172.98±42.56)pg/ml were lower than the healthy control group(1.62±0.44)pg/ml,(204.23±48.88)pg/ml.The above differences were statistically significant(P<0.05).Conclusions The imbalance of Th1/Th2 and Th17/Treg cytokines may be one of the important immunological pathogenesis of CA.Systemic cell-mediated immunity dysfunction may exist in CA patients.