色素上皮源性因子、内质网氨基肽酶1和Sprouty1在银屑病皮损组织中的表达及相关性研究

Expression and correlation of pigment epidermal derived factor,endoplasmic reticulum aminopeptidase 1 and Sprouty1 in psoriatic lesions

目的研究银屑病皮损组织中色素上皮源性因子(PEDF)、表皮内质网氨基肽酶1(ERAP1)和Sprouty1的表达情况及相关性。方法收集河北省儿童医院皮肤科病理室2019年1月—2020年12月诊断的120例银屑病患者皮损组织蜡块标本进行研究,并纳入病例组;另外选取该院实验室保存的120例健康体检者的皮肤组织蜡块标本作为对照,纳入健康对照组。对比两组PEDF、ERAP1和Sprouty1的表达差异,单因素分析银屑病患者不同病情严重程度的PEDF、ERAP1和Sprouty1表达差异,分析PEDF、ERAP1和Sprouty1与银屑病患者皮损严重程度的相关性。结果①与健康对照组相比,病例组的PEDF、ERAP1 mRNA和蛋白均呈高表达,Sprouty1 mRNA和蛋白均呈低表达(P<0.05);②重度银屑病患者皮损的PEDF、ERAP1表达明显高于轻中度患者,Sprouty1表达明显低于轻中度患者(P<0.05);③PEDF、ERAP1的表达均与银屑病皮损面积严重度指数(PASI)评分呈正相关,Sprouty1的表达与PASI评分呈负相关。结论银屑病患者皮损组织中PEDF、ERAP1和Sprouty1的表达与正常组织存在明显差异,PEDF、ERAP1和Sprouty1的表达均与银屑病皮损组织严重程度存在密切关系。在银屑病患者治疗以及诊断中可将PEDF、ERAP1和Sprouty1作为新的研究方向进行深入探索。

Objective To study the expression and correlation of PEDF,ERAP1 and Sprouty1 in psoriatic lesions.Methods 120 patients with psoriasis diagnosed in the pathology department of dermatology of Children's Hospital of Hebei Province from January 2019 to December 2020 were included in the case group for the study.In addition,120 healthy patients without psoriasis were selected from the laboratory of this hospital for the control group.The expression differences of PEDF,ERAP1 and Sprouty1 in the two groups were compared,and the expression differences of PEDF,ERAP1 and Sprouty1 in patients with psoriasis with different severity were analyzed by univariate analysis,and the correlation between PEDF,ERAP1 and Sprouty1 and the severity of psoriasis was analyzed.Results①Compared with healthy control group,PEDF and ERAP1 mRNA and protein were highly expressed in case group,while Sprouty1 mRNA and protein were low expression(P<0.05);②The expression of PEDF and ERAP1 in patients with severe psoriasis significantly increased than that in patients with mild to moderate psoriasis,and the expression of Sprouty1 significantly decreased than that in patients with mild to moderate psoriasis(P<0.05);③The expression of PEDF and ERAP1 were positively correlated with psoriatic PASI score,while the expression of Sprouty1 was negatively correlated with psoriatic PASI score.Conclusions The expressions of PEDF,ERAP1 and Sprouty1 in psoriasis patients are significantly different from those in normal tissues,and the expressions of PEDF,ERAP1 and Sprouty1 are closely related to the severity of psoriasis.PEDF,ERAP1 and Sprouty1 can be further explored in the treatment and diagnosis of psoriasis patients.