PRR11在老年肺癌组织中的表达及其与肿瘤分化程度和TNM分期的关系研究

Expression of PRR11 in elderly lung cancer and its relationship with tumor differentiation and TNM staging

目的研究富脯氨酸蛋白11(PRR11)在老年肺癌组织中的表达及其与肿瘤分化程度和TNM分期的关系。方法采用回顾性研究,收集65例老年(年龄≥60岁)肺癌组织标本及其癌旁组织标本,另收集55例正常肺部组织标本作为对照。采用免疫印迹法对不同组织标本中PRR11的表达水平进行检测,观察PRR11表达水平与肿瘤分化程度和TNM分期的相关性。结果在老年肺癌组织标本中,PRR11阴性15例(23.08%),阳性50例(76.92%)。PRR11蛋白在老年肺癌组织标本中的阳性率较癌旁组织(6.15%)显著升高(χ2=64.14,P<0.01),且较正常肺部组织标本的阳性率(0.00%)亦显著升高(P<0.01),而癌旁组织与正常肺部组织标本中PRR11蛋白阳性率的比较,并无显著差异(P>0.05)。经免疫印迹法检测结果发现,PRR11蛋白在老年肺癌组织标本中的表达水平为2.21±0.34,较癌旁组(1.00±0.19)、正常肺部组织的表达水平(0.93±0.15)均显著升高(t=15.65,13.96,P<0.01),癌旁组织与正常肺部组织标本中PRR11蛋白表达水平的比较,无显著差异(t=0.45,P>0.05)。在老年肺癌组织标本中,不同性别、年龄、肿瘤直径、病理类型、是否伴有淋巴结转移的患者PRR11蛋白表达阳性率比较,并无显著差异(χ~2=2.74,0.95,0.66,2.26,0.08,均P>0.05);而肿瘤低分化、TNM分期为Ⅲa的患者PRR11蛋白表达阳性率明显高于肿瘤中高分化、TNM分期为Ⅰa~Ⅱb者,差异具有统计学意义(χ~2=17.04,27.64,均P<0.05)。结论PRR11蛋白在老年肺癌组织标本中具有高表达的特点,且其表达水平与患者TNM分期、肿瘤分化程度均密切相关。

Objective To study the expression of proline-rich protein 11(PRR11)in elderly lung cancer and its relationship with the degree of differentiation and TNM stage.Methods A retrospective study was conducted to collect 65 lung cancer tissue specimens and adjacent tissue specimens from elderly patients(aged over 60 years)and 55 normal lung tissue specimens as controls.The expression of PRR11 in different tissues was detected by Western blotting.Results Among the elderly lung cancer tissue specimens,PRR11 was negative in 15 cases(23.08%)and positive in 50 cases(76.92%).The positive rate of PRR11 protein in elderly lung cancer tissues was significantly higher than that in adjacent lung tissues(6.15%)(χ~2=64.14,P<0.01),and it was also significantly higher than that in normal lung tissues(0.00%)(P<0.01).There was no significant difference in the positive rate of PRR11 protein between adjacent lung tissues and normal lung tissues(P>0.05).The expression level of PRR11 protein in lung cancer tissues of the elderly was(2.21±0.34),which was significantly higher than that in adjacent lung tissues(1.00±0.19)and normal lung tissues(0.93±0.15)(t=15.65,13.96,both P<0.01).The expression level of PRR11 protein in adjacent lung tissues and normal lung tissues was significantly higher than that in adjacent lung tissues(t=15.65,13.96,both P<0.01).There was no significant difference in expression level(t=0.45,P>0.05).There was no significant difference in the positive rate of PRR11 protein expression among different sex,age,tumor diameter,pathological type and lymph node metastasis(χ~2=2.74,0.95,0.66,2.26,0.08,all P>0.05).The PRR11 protein expression was positive in patients with different TNM stages and tumor differentiation,with significant difference(χ~2=17.04,27.64,both P<0.05).Conclusion PRR11 protein is highly expressed in lung cancer tissues of elderly patients,and its expression level is closely related to TNM staging and tumor differentiation.